Xia Bing, Wang Bin, Shi Jisen, Zhang Yu, Zhang Qi, Chen Zhenyu, Li Jiachen
Key Laboratory of Forest Genetics & Biotechnology (Ministry of Education of China), Nanjing Forestry University, Nanjing 210037, PR China; Advanced Analysis & Testing Center, College of Science, Nanjing Forestry University, Nanjing 210037, PR China.
Advanced Analysis & Testing Center, College of Science, Nanjing Forestry University, Nanjing 210037, PR China.
Acta Biomater. 2017 Mar 15;51:197-208. doi: 10.1016/j.actbio.2017.01.015. Epub 2017 Jan 6.
To develop photothermal and biodegradable nanocarriers for combined chemo-photothermal therapy of cancer, polyaniline/porous silicon hybrid nanocomposites had been successfully fabricated via surface initiated polymerization of aniline onto porous silicon nanoparticles in our experiments. As-prepared polyaniline/porous silicon nanocomposites could be well dispersed in aqueous solution without any extra hydrophilic surface coatings, and showed a robust photothermal effect under near-infrared (NIR) laser irradiation. Especially, after an intravenous injection into mice, these biodegradable porous silicon-based nanocomposites as non-toxic agents could be completely cleared in body. Moreover, these polyaniline/porous silicon nanocomposites as drug carriers also exhibited an efficient loading and dual pH/NIR light-triggered release of doxorubicin hydrochloride (DOX, a model anticancer drug). Most importantly, assisted with NIR laser irradiation, polyaniline/PSiNPs nanocomposites with loading DOX showed a remarkable synergistic anticancer effect combining chemotherapy with photothermal therapy, whether in vitro or in vivo. Therefore, based on biodegradable PSiNPs-based nanocomposites, this combination approach of chemo-photothermal therapy would have enormous potential on clinical cancer treatments in the future.
Considering the non-biodegradable nature and potential long-term toxicity concerns of photothermal nanoagents, it is of great interest and importance to develop biodegradable and photothermal nanoparticles with an excellent biocompatibility for their future clinical applications. In our experiments, we fabricated porous silicon-based hybrid nanocomposites via surface initiated polymerization of aniline, which showed an excellent photothermal effect, aqueous dispersibility, biodegradability and biocompatibility. Furthermore, after an efficient loading of DOX molecules, polyaniline/porous silicon nanocomposites exhibited the remarkable synergistic anticancer effect, whether in vitro and in vivo.
为开发用于癌症联合化疗-光热疗法的光热可生物降解纳米载体，在我们的实验中，通过将苯胺在多孔硅纳米颗粒上进行表面引发聚合，成功制备了聚苯胺/多孔硅杂化纳米复合材料。所制备的聚苯胺/多孔硅纳米复合材料无需任何额外的亲水性表面涂层即可在水溶液中良好分散，并在近红外（NIR）激光照射下表现出强大的光热效应。特别是，将这些可生物降解的多孔硅基纳米复合材料作为无毒剂静脉注射到小鼠体内后，可在体内完全清除。此外，这些聚苯胺/多孔硅纳米复合材料作为药物载体还表现出对盐酸多柔比星（DOX，一种抗癌模型药物）的高效负载以及双pH/NIR光触发释放。最重要的是，在NIR激光照射辅助下，负载DOX的聚苯胺/多孔硅纳米复合材料无论是在体外还是体内都显示出化疗与光热疗法相结合的显著协同抗癌效果。因此，基于可生物降解的多孔硅基纳米复合材料，这种化疗-光热疗法的联合方法在未来临床癌症治疗中具有巨大潜力。
考虑到光热纳米剂的不可生物降解性质以及潜在的长期毒性问题，开发具有优异生物相容性的可生物降解光热纳米颗粒以用于其未来临床应用具有极大的兴趣和重要性。在我们的实验中，通过苯胺的表面引发聚合制备了多孔硅基杂化纳米复合材料，其表现出优异的光热效应、水分散性、生物降解性和生物相容性。此外，在高效负载DOX分子后，聚苯胺/多孔硅纳米复合材料无论是在体外还是体内都表现出显著的协同抗癌效果。
