Liu Zhijun, Long Xueying, Liu Hui
Department of Radiology, First Affliated Hospital of University of South China, Hengyang Hunan 421001, China.
Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Dec 28;41(12):1270-1277. doi: 10.11817/j.issn.1672-7347.2016.12.004.
To investigate the correlations among total liver CT perfusion parameters, unpaired arteries (UAs) and microvessel area (MVA) in a rabbit liver VX2 tumor model, and to learn the tumoral angiogenesis condition and the mechanisms for perfusion imaging. Methods: Rabbits with or without the inoculated VX2 tumor in the liver underwent total liver CT perfusion imaging 2 weeks after the operation. Perfusion parameters included blood flow (BF), blood volume (BV), arterial liver perfusion (ALP), portal liver perfusion (PVP), hepatic perfusion index (HPI) for the tumor rim and the surrounding liver tissue. After the examination, the UAs and MVA of tumor tissues were obtained by immunohistochemical staining. The differences of perfusion parameters between the vital tumor rim and the surrounding liver tissue were compared. The correlations among perfusion parameters, UAs and MVA were analyzed. Results: There was significant difference between the CT perfusion parameters at the tumor rim and the surrounding liver tissue or liver tissue of the control group (P<0.05), but there was no significant difference between the perfusion parameters at the surrounding liver tissues of the experimental group and the control (P>0.05). There was positive correlation between UAs and MVA. UAs and MVA were positively correlated with BF, ALP and BV at the tumor rim. UAs and MVA were negatively correlated with PVP. HPI positively correlated with UAs, but it was not correlated with MVA. Conclusion: Total liver CT perfusion can provide quantitative information to evaluate the artery and portal vein perfusion of liver VX2 tumor, and to assess the degree of tumor angiogenesis.
探讨兔肝VX2肿瘤模型中肝脏CT灌注参数、非配对动脉（UAs）与微血管面积（MVA）之间的相关性，了解肿瘤血管生成情况及灌注成像机制。方法：对肝内接种或未接种VX2肿瘤的兔子在术后2周进行全肝CT灌注成像。灌注参数包括肿瘤边缘及周围肝组织的血流量（BF）、血容量（BV）、肝动脉灌注（ALP）、门静脉灌注（PVP）、肝灌注指数（HPI）。检查后，通过免疫组化染色获取肿瘤组织的UAs和MVA。比较肿瘤活性边缘与周围肝组织灌注参数的差异。分析灌注参数、UAs和MVA之间的相关性。结果：肿瘤边缘与周围肝组织或对照组肝组织的CT灌注参数有显著差异（P<0.05），但实验组周围肝组织与对照组的灌注参数无显著差异（P>0.05）。UAs与MVA呈正相关。UAs和MVA与肿瘤边缘的BF、ALP和BV呈正相关。UAs和MVA与PVP呈负相关。HPI与UAs呈正相关，但与MVA无相关性。结论：全肝CT灌注可为评估肝VX2肿瘤的动脉和门静脉灌注及肿瘤血管生成程度提供定量信息。
