Xiao Rong, Wu Hai-Long, Hu Yong, Yin Xiao-Li, Gu Hui-Wen, Liu Zhi, Wang Tong, Sun Xiao-Dong, Yu Ru-Qin
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University.
Anal Sci. 2017;33(1):29-34. doi: 10.2116/analsci.33.29.
In the present work, a practical method that combines excitation-emission matrix fluorescence with a second-order calibration method based on an alternating trilinear decomposition (ATLD) algorithm was developed in order to simultaneously and directly determine the contents of warfarin (WAR) and aspirin (ASA) in human plasma and urine samples, even in the presence of unknown interferences. With the pre-estimated component number of 4, the obtained average spiked recoveries were 105.4 ± 7.8 and 104.2 ± 8.3% for WAR, 96.5 ± 2.8 and 91.2 ± 2.3% for ASA in human plasma and urine samples, respectively. Furthermore, the figures of merit were calculated and also inter- and intra-day experiments were performed that proved the proposed method is of great significance to the monitoring of clinical administration and also being a simple sample pretreatment at low-cost.
在本研究中，开发了一种将激发-发射矩阵荧光与基于交替三线性分解（ATLD）算法的二阶校准方法相结合的实用方法，以便在存在未知干扰的情况下，同时直接测定人体血浆和尿液样本中华法林（WAR）和阿司匹林（ASA）的含量。在预估计组分数为4的情况下，在人体血浆和尿液样本中，WAR的加标平均回收率分别为105.4±7.8%和104.2±8.3%，ASA的加标平均回收率分别为96.5±2.8%和91.2±2.3%。此外，还计算了品质因数，并进行了日间和日内实验，结果证明所提出的方法对于临床用药监测具有重要意义，并且是一种低成本的简单样品预处理方法。
