Salmani Izadi Mohammad, Naserian Abbas Ali, Nasiri Mohammad Reza, Majidzadeh Heravi Reza, Valizadeh Reza
Department of Animal Sciences, Ferdowsi University of Mashhad, International Campus, Mashhad, Iran.
Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Rep Biochem Mol Biol. 2016 Oct;5(1):33-39.
With the increasing concern for health and nutrition, dietary fat has attracted considerable attention. The composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. The fatty acid synthase (FASN) and stearoyl-CoA desaturase (delta-9-desaturase) (SCD) genes affect fatty acid composition (1). The expression of SCD and FASN genes is related to an increase in conjugated linoleic acid (CLA) in dairy products, which benefits human health.The aim of current study was to investigate expression changes of SCD and FASN genes that resulted from crossbreeding the local Baluchi sheep with alien breeds.
We collected tissue samples from the mammary glands of 24 single-born ewes from local Baluchi and synthetic Iran-Black and Arman sheep breeds in the Abbas Abad breeding center. After RNA extraction and cDNA synthesis, real-time PCR was performed with all samples in triplicate.
The maximum and minimum expression of SCD and FASN genes was in the local Baluchi sheep and the crossbred Arman sheep, respectively.
With the highest SCD and FASN gene expression in local Baluchi sheep and relatively less expression of these genes in synthetic Iran-Black and Arman Sheep breeds, it may be necessary to consider the consequences of crossbreeding local sheep and the fatty acid composition of their dairy products.
随着对健康和营养的关注度不断提高，膳食脂肪已引起相当大的关注。饮食中脂肪酸的组成很重要，因为它们与包括癌症、糖尿病和心血管疾病在内的主要疾病有关。脂肪酸合酶（FASN）和硬脂酰辅酶A去饱和酶（δ-9-去饱和酶）（SCD）基因会影响脂肪酸组成（1）。SCD和FASN基因的表达与乳制品中共轭亚油酸（CLA）的增加有关，CLA对人类健康有益。本研究的目的是调查当地俾路支羊与外来品种杂交导致的SCD和FASN基因表达变化。
我们从阿巴斯阿巴德育种中心的当地俾路支羊以及合成的伊朗黑羊和阿尔曼羊品种的24只单胎母羊的乳腺中采集了组织样本。提取RNA并合成cDNA后，对所有样本进行实时PCR，一式三份。
SCD和FASN基因的最高和最低表达分别出现在当地俾路支羊和杂交的阿尔曼羊中。
鉴于当地俾路支羊中SCD和FASN基因表达最高，而在合成的伊朗黑羊和阿尔曼羊品种中这些基因的表达相对较少，可能有必要考虑当地绵羊杂交的后果及其乳制品的脂肪酸组成。
