包含……基因的克隆载体的设计与构建
Design and Construction of a Cloning Vector Containing the Gene of .
作者信息
Yaghoubi Atieh, Aryan Ehsan, Zare Hosna, Alami Shadi, Teimourpour Roghayeh, Meshkat Zahra
机构信息
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
出版信息
Rep Biochem Mol Biol. 2016 Oct;5(1):46-50.
BACKGROUND
Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the .
METHODS
First, an fragment was amplified by PCR and cloned into plasmid pcDNA3.1(+) and recombinant vector was confirmed.
RESULTS
A 435 bp fragment was isolated. The fragment was 100% homologous with of M. tuberculosis strain H37Rv in GenBank.
CONCLUSION
In this study, the cloning vector pcDNA3.1(+), containing a 435-bp fragment of , was constructed. This could be used as a DNA vaccine to induce immune responses in animal models in future studies.
背景
结核病是全球主要的死亡原因。找到一种有效的抗结核疫苗是控制该病的最佳方法。已经研发了几种针对这种疾病的疫苗，但没有一种具有完全的保护作用。本研究的目的是设计并构建一个包含……的克隆载体。
方法
首先，通过聚合酶链反应（PCR）扩增一个……片段，并将其克隆到质粒pcDNA3.1(+)中，然后对重组载体进行确认。
结果
分离出一个435 bp的……片段。该片段与GenBank中结核分枝杆菌H37Rv菌株的……100%同源。
结论
在本研究中，构建了包含435 bp……片段的克隆载体pcDNA3.1(+)。在未来的研究中，这可作为一种DNA疫苗在动物模型中诱导免疫反应。
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