Venediktova Natalya I, Gorbacheva Olga S, Belosludtseva Natalia V, Fedotova Irina B, Surina Natalia M, Poletaeva Inga I, Kolomytkin Oleg V, Mironova Galina D
Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia.
Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia.
J Bioenerg Biomembr. 2017 Apr;49(2):149-158. doi: 10.1007/s10863-016-9693-5. Epub 2017 Jan 9.
The role of brain and liver mitochondria at epileptic seizure was studied on Krushinsky-Molodkina (KM) rats which respond to sound with an intensive epileptic seizure (audiogenic epilepsy). We didn't find significant changes in respiration rats of brain and liver mitochondria of KM and control rats; however the efficiency of АТР synthesis in the KM rat mitochondria was 10% lower. In rats with audiogenic epilepsy the concentration of oxidative stress marker malondialdehyde in mitochondria of the brain (but not liver) was 2-fold higher than that in the control rats. The rate of HO generation in brain mitochondria of КМ rats was twofold higher than in the control animals when using NAD-dependent substrates. This difference was less pronounced in liver mitochondria. In KM rats, the activity of mitochondrial ATP-dependent potassium channel was lower than in liver mitochondria of control rats. The comparative study of the mitochondria ability to retain calcium ions revealed that in the case of using the complex I and complex II substrates, permeability transition pore is easier to trigger in brain and liver mitochondria of KM and КМs rats than in the control ones. The role of the changes in the energetic, oxidative, and ionic exchange in the mechanism of audiogenic epilepsy generation in rats and the possible correction of the epilepsy seizures are discussed.
在对声音产生强烈癫痫发作（听源性癫痫）的克鲁申斯基-莫洛金娜（KM）大鼠身上，研究了大脑和肝脏线粒体在癫痫发作中的作用。我们未发现KM大鼠和对照大鼠大脑及肝脏线粒体呼吸方面的显著变化；然而，KM大鼠线粒体中ATP合成效率低10%。在听源性癫痫大鼠中，大脑（而非肝脏）线粒体中氧化应激标志物丙二醛的浓度比对照大鼠高2倍。当使用NAD依赖性底物时，KM大鼠大脑线粒体中HO的生成速率比对照动物高2倍。这种差异在肝脏线粒体中不太明显。在KM大鼠中，线粒体ATP依赖性钾通道的活性低于对照大鼠的肝脏线粒体。对线粒体保留钙离子能力的比较研究表明，在使用复合体I和复合体II底物的情况下，KM大鼠和KM s大鼠大脑及肝脏线粒体比对照大鼠更容易触发通透性转换孔。讨论了能量、氧化和离子交换变化在大鼠听源性癫痫发作机制中的作用以及癫痫发作可能的纠正方法。
