Delapierre François-Damien, Mottet Guillaume, Taniga Vélan, Boisselier Julie, Viovy Jean-Louis, Malaquin Laurent
Physico-Chimie Curie, Institut Curie, PSL Research University, Centre National de Recherche Scientifique (CNRS), UMR 168, Université Pierre et Marie Curie (UPMC), F-75005, Paris, France. Institut Pierre-Gilles de Gennes, F-75005, Paris, France.
Biofabrication. 2017 Feb 6;9(1):015015. doi: 10.1088/1758-5090/aa5852.
A novel technology is reported to immobilize different types of particles or cells on a surface at predefined positions with a micrometric precision. The process uses capillary assembly on arrays of crescent-shaped structures with different orientations. Sequential assemblies in different substrate orientations with different types of particles allow for the creation of imbricated and multiplexed arrays. In this work up to four different types of particles were deterministically localized on a surface. Using this process, antibody coated microparticles were assembled on substrates and used as capture patterns for the creation of complex cell networks. This new technology may have numerous applications in biology, e.g. for fast cell imaging, cell-cell interactions studies, or construction of cell arrays.
据报道，一种新技术可将不同类型的颗粒或细胞以微米级精度固定在表面的预定位置。该过程利用在具有不同取向的新月形结构阵列上的毛细管组装。在不同的底物取向上使用不同类型的颗粒进行顺序组装，可以创建交错和多重阵列。在这项工作中，多达四种不同类型的颗粒被确定性地定位在一个表面上。利用这一过程，将抗体包被的微粒组装在底物上，并用作创建复杂细胞网络的捕获模式。这项新技术在生物学中可能有许多应用，例如用于快速细胞成像、细胞间相互作用研究或细胞阵列构建。
