Hacohen Adar, Jessel Hadass R, Richter-Levin Alon, Shefi Orit
The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel.
The Faculty of Engineering, Bar Ilan University, Ramat Gan 5290002, Israel.
Micromachines (Basel). 2020 May 15;11(5):505. doi: 10.3390/mi11050505.
The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most of them deal with the patterning of cell populations, and are either not suitable or difficult to implement for the patterning of single cells. Here, we describe a bottom-up strategy for the micropatterning of cells and cell-sized particles. We have configured a micromanipulator system, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from different types, and positioning them with high accuracy in a predefined position, with a resolution smaller than 10 µm. Complementary DNA sequences were used to stabilize and hold the patterns together. The system is accurate, flexible, and easy-to-use, and can be automated for larger-scale tasks. Importantly, it maintains the viability of live cells. We provide quantitative measurements of the process and offer a file format for such assemblies.
将细胞操纵并选择性地定位成特定模式或置于不同微环境中的能力，是许多单细胞实验方法和生物工程应用的重要组成部分。尽管已经展示了多种粒子和细胞图案化方法，但其中大多数处理的是细胞群体的图案化，对于单细胞图案化而言，要么不适用，要么难以实施。在此，我们描述一种用于细胞和细胞大小粒子微图案化的自下而上策略。我们构建了一个微操纵系统，其中一个气动微注射器与一个持液吸管相连，该持液吸管能够从不同类型中物理分离单个粒子和细胞，并将它们高精度地定位在预定义位置，分辨率小于10微米。互补DNA序列用于稳定图案并将其固定在一起。该系统准确、灵活且易于使用，并且可以针对大规模任务实现自动化。重要的是，它能维持活细胞的活力。我们提供了该过程的定量测量，并为此类组装提供了一种文件格式。
