• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

三细胞紧密连接蛋白 LSR 的缺失通过上调人子宫内膜癌细胞中的 TEAD1/AREG 促进细胞侵袭和迁移。

Loss of tricellular tight junction protein LSR promotes cell invasion and migration via upregulation of TEAD1/AREG in human endometrial cancer.

机构信息

Departments of Obstetrics and Gynecology, University School of Medicine, Sapporo, Japan.

Department of Cell Science, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.

出版信息

Sci Rep. 2017 Jan 10;7:37049. doi: 10.1038/srep37049.

DOI:10.1038/srep37049
PMID:28071680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5223122/
Abstract

Lipolysis-stimulated lipoprotein receptor (LSR) is a unique molecule of tricellular contacts of normal and cancer cells. We investigated how the loss of LSR induced cell migration, invasion and proliferation in endometrial cancer cell line Sawano. mRNAs of amphiregulin (AREG) and TEA domain family member 1 (TEAD1) were markedly upregulated by siRNA-LSR. In endometrial cancer tissues, downregulation of LSR and upregulation of AREG were observed together with malignancy, and Yes-associated protein (YAP) was present in the nuclei. siRNA-AREG prevented the cell migration and invasion induced by siRNA-LSR, whereas treatment with AREG induced cell migration and invasion. LSR was colocalized with TRIC, angiomotin (AMOT), Merlin and phosphorylated YAP (pYAP). siRNA-LSR increased expression of pYAP and decreased that of AMOT and Merlin. siRNA-YAP prevented expression of the mRNAs of AREG and TEAD1, and the cell migration and invasion induced by siRNA-LSR. Treatment with dobutamine and 2-deoxy-D-glucose and glucose starvation induced the pYAP expression and prevented the cell migration and invasion induced by siRNA-LSR. siRNA-AMOT decreased the Merlin expression and prevented the cell migration and invasion induced by siRNA-LSR. The loss of LSR promoted cell invasion and migration via upregulation of TEAD1/AREG dependent on YAP/pYAP and AMOT/Merlin in human endometrial cancer cells.

摘要

脂肪酶刺激的脂蛋白受体（LSR）是正常和癌细胞三细胞接触的独特分子。我们研究了 LSR 缺失如何诱导子宫内膜癌细胞系 Sawano 中的细胞迁移、侵袭和增殖。用 siRNA-LSR 显著上调了 Amphiregulin（AREG）和 TEA 结构域家族成员 1（TEAD1）的 mRNA。在子宫内膜癌组织中，LSR 的下调和 AREG 的上调与恶性肿瘤一起观察到，Yes 相关蛋白（YAP）存在于核内。siRNA-AREG 可预防 siRNA-LSR 诱导的细胞迁移和侵袭，而 AREG 处理则诱导细胞迁移和侵袭。LSR 与 TRIC、血管运动蛋白（AMOT）、 Merlin 和磷酸化 YAP（pYAP）共定位。siRNA-LSR 增加了 pYAP 的表达，减少了 AMOT 和 Merlin 的表达。siRNA-YAP 可预防 siRNA-LSR 诱导的 AREG 和 TEAD1 mRNA 的表达以及细胞迁移和侵袭。多巴酚胺和 2-脱氧-D-葡萄糖处理以及葡萄糖饥饿诱导 pYAP 表达，并阻止 siRNA-LSR 诱导的细胞迁移和侵袭。siRNA-AMOT 降低 Merlin 表达，并阻止 siRNA-LSR 诱导的细胞迁移和侵袭。LSR 的缺失通过 YAP/pYAP 和 AMOT/Merlin 依赖性上调 TEAD1/AREG 促进了人子宫内膜癌细胞的侵袭和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/3c6ec82dccbd/srep37049-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/4339e8808611/srep37049-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/41d20d8e5a93/srep37049-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/fd318c7a6068/srep37049-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/6c6a7e4e631f/srep37049-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/9460db04429c/srep37049-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/19595cea6bc5/srep37049-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/3c6ec82dccbd/srep37049-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/4339e8808611/srep37049-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/41d20d8e5a93/srep37049-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/fd318c7a6068/srep37049-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/6c6a7e4e631f/srep37049-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/9460db04429c/srep37049-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/19595cea6bc5/srep37049-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12be/5223122/3c6ec82dccbd/srep37049-f7.jpg

相似文献

1
Loss of tricellular tight junction protein LSR promotes cell invasion and migration via upregulation of TEAD1/AREG in human endometrial cancer.三细胞紧密连接蛋白 LSR 的缺失通过上调人子宫内膜癌细胞中的 TEAD1/AREG 促进细胞侵袭和迁移。
Sci Rep. 2017 Jan 10;7:37049. doi: 10.1038/srep37049.
2
ASPP2 suppression promotes malignancy via LSR and YAP in human endometrial cancer.ASPP2 通过 LSR 和 YAP 抑制促进人子宫内膜癌的恶性转化。
Histochem Cell Biol. 2020 Aug;154(2):197-213. doi: 10.1007/s00418-020-01876-8. Epub 2020 Apr 7.
3
The roles of tricellular tight junction protein lipolysis-stimulated lipoprotein receptor in malignancy of human endometrial cancer cells.三细胞紧密连接蛋白脂解刺激脂蛋白受体在人子宫内膜癌细胞恶性肿瘤中的作用。
Oncotarget. 2016 May 10;7(19):27735-52. doi: 10.18632/oncotarget.8408.
4
Tricellular tight junction protein LSR/angulin-1 contributes to the epithelial barrier and malignancy in human pancreatic cancer cell line.三细胞紧密连接蛋白 LSR/angulin-1 有助于人胰腺癌细胞系的上皮屏障和恶性转化。
Histochem Cell Biol. 2020 Jan;153(1):5-16. doi: 10.1007/s00418-019-01821-4. Epub 2019 Oct 24.
5
Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells.细胞分裂过程中人类上皮细胞中中层和中心体处的三联细胞紧密连接分子 LSR 的定位。
J Histochem Cytochem. 2020 Jan;68(1):59-72. doi: 10.1369/0022155419886263. Epub 2019 Oct 29.
6
Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.脂解刺激脂蛋白受体的下调通过紧密连接蛋白-1介导的基质金属蛋白酶促进人子宫内膜癌的细胞侵袭。
Oncol Lett. 2017 Dec;14(6):6776-6782. doi: 10.3892/ol.2017.7038. Epub 2017 Sep 22.
7
Role of Tricellular Tight Junction Protein Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Cancer Cells.三细胞紧密连接蛋白脂肪酶刺激的脂蛋白受体（LSR）在癌细胞中的作用。
Int J Mol Sci. 2019 Jul 20;20(14):3555. doi: 10.3390/ijms20143555.
8
The roles of tight junction protein cingulin in human endometrioid endometrial cancer.紧密连接蛋白cingulin在人子宫内膜样腺癌中的作用
Tissue Barriers. 2024 Jun 2:2361976. doi: 10.1080/21688370.2024.2361976.
9
Angiomotin promotes breast cancer cell proliferation and invasion.血管动蛋白促进乳腺癌细胞的增殖和侵袭。
Oncol Rep. 2015 Apr;33(4):1938-46. doi: 10.3892/or.2015.3780. Epub 2015 Feb 3.
10
The Roles of Tricellular Tight Junction Protein Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Endometriosis and Endometrioid-Endometrial Carcinoma.三细胞紧密连接蛋白Angulin-1/脂肪分解刺激脂蛋白受体（LSR）在子宫内膜异位症和子宫内膜样子宫内膜癌中的作用
Cancers (Basel). 2021 Dec 17;13(24):6341. doi: 10.3390/cancers13246341.

引用本文的文献

1
Defining the Molecular Intricacies of Human Papillomavirus-Associated Tonsillar Carcinoma.定义人乳头瘤病毒相关扁桃体癌的分子复杂性。
Cancer Control. 2025 Jan-Dec;32:10732748241310932. doi: 10.1177/10732748241310932. Epub 2025 May 7.
2
The interplay between the epithelial permeability barrier, cell migration and mitochondrial metabolism of growth factors and their inhibitors in a human endometrial carcinoma cell line.上皮通透性屏障、细胞迁移以及生长因子和其抑制剂的线粒体代谢之间的相互作用在人子宫内膜癌细胞系中的研究。
Tissue Barriers. 2024 Oct;12(4):2304443. doi: 10.1080/21688370.2024.2304443. Epub 2024 Jan 15.
3

本文引用的文献

1
Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.脂解刺激脂蛋白受体的下调通过紧密连接蛋白-1介导的基质金属蛋白酶促进人子宫内膜癌的细胞侵袭。
Oncol Lett. 2017 Dec;14(6):6776-6782. doi: 10.3892/ol.2017.7038. Epub 2017 Sep 22.
2
The roles of tricellular tight junction protein lipolysis-stimulated lipoprotein receptor in malignancy of human endometrial cancer cells.三细胞紧密连接蛋白脂解刺激脂蛋白受体在人子宫内膜癌细胞恶性肿瘤中的作用。
Oncotarget. 2016 May 10;7(19):27735-52. doi: 10.18632/oncotarget.8408.
3
Metastasis to Bone in Human Cancer Is Associated with Loss of Occludin Expression.
Unraveling the Molecular Puzzle: Exploring Gene Networks across Diverse EMT Status of Cell Lines.
解析分子谜题：探索不同 EMT 状态细胞系中的基因网络。
Int J Mol Sci. 2023 Aug 14;24(16):12784. doi: 10.3390/ijms241612784.
4
Downregulation of angulin-1/LSR induces malignancy via upregulation of EGF-dependent claudin-2 and TGF-β-dependent cell metabolism in human lung adenocarcinoma A549 cells.下调角蛋白聚糖-1/LSR 通过上调表皮生长因子依赖性紧密连接蛋白-2 和 TGF-β依赖性细胞代谢诱导人肺腺癌细胞 A549 的恶性转化。
Oncotarget. 2023 Mar 24;14:261-275. doi: 10.18632/oncotarget.27728.
5
Atypical Macropinocytosis Contributes to Malignant Progression: A Review of Recent Evidence in Endometrioid Endometrial Cancer Cells.非典型巨吞饮作用促进恶性进展：子宫内膜样子宫内膜癌细胞近期证据综述
Cancers (Basel). 2022 Oct 15;14(20):5056. doi: 10.3390/cancers14205056.
6
ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma.ZEB2/TWIST1/PRMT5/核小体重塑去乙酰化酶多复合体参与结直肠癌上皮-间质转化和转移的表观遗传调控。
Cancers (Basel). 2022 Jul 14;14(14):3426. doi: 10.3390/cancers14143426.
7
TEADs serve as potential prognostic biomarkers and targets for human gastric cancer.TEADs 可作为人类胃癌的潜在预后生物标志物和靶点。
BMC Gastroenterol. 2022 Jun 24;22(1):308. doi: 10.1186/s12876-022-02386-8.
8
Lipolysis-Stimulated Lipoprotein Receptor Impairs Hepatocellular Carcinoma and Inhibits the Oncogenic Activity of YAP1 PPPY Motif.脂解刺激脂蛋白受体损害肝细胞癌并抑制YAP1 PPPY基序的致癌活性。
Front Oncol. 2022 May 2;12:896412. doi: 10.3389/fonc.2022.896412. eCollection 2022.
9
LncRNA-DANCR promotes growth and metastasis of colorectal cancer via activating epithelial-mesenchymal transition process.长链非编码RNA-DANCR通过激活上皮-间质转化过程促进结直肠癌的生长和转移。
Transl Cancer Res. 2019 Nov;8(7):2517-2525. doi: 10.21037/tcr.2019.10.09.
10
The Roles of Tricellular Tight Junction Protein Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Endometriosis and Endometrioid-Endometrial Carcinoma.三细胞紧密连接蛋白Angulin-1/脂肪分解刺激脂蛋白受体（LSR）在子宫内膜异位症和子宫内膜样子宫内膜癌中的作用
Cancers (Basel). 2021 Dec 17;13(24):6341. doi: 10.3390/cancers13246341.
人类癌症中的骨转移与闭合蛋白表达缺失有关。
Anticancer Res. 2016 Mar;36(3):1287-93.
4
Loss of tumor suppressor Merlin results in aberrant activation of Wnt/β-catenin signaling in cancer.肿瘤抑制因子Merlin的缺失会导致癌症中Wnt/β-连环蛋白信号通路的异常激活。
Oncotarget. 2016 Apr 5;7(14):17991-8005. doi: 10.18632/oncotarget.7494.
5
Loss of CAR promotes migration and proliferation of HaCaT cells, and accelerates wound healing in rats via Src-p38 MAPK pathway.CAR的缺失促进了HaCaT细胞的迁移和增殖，并通过Src-p38丝裂原活化蛋白激酶途径加速了大鼠的伤口愈合。
Sci Rep. 2016 Jan 25;6:19735. doi: 10.1038/srep19735.
6
Hippo Pathway in Organ Size Control, Tissue Homeostasis, and Cancer.器官大小调控、组织稳态及癌症中的河马信号通路
Cell. 2015 Nov 5;163(4):811-28. doi: 10.1016/j.cell.2015.10.044.
7
Hippo pathway elements Co-localize with Occludin: A possible sensor system in pancreatic epithelial cells.河马通路元件与闭合蛋白共定位：胰腺上皮细胞中一种可能的传感系统。
Tissue Barriers. 2015 May 1;3(3):e1037948. doi: 10.1080/21688370.2015.1037948. eCollection 2015 Jul-Sep.
8
A role for the transducer of the Hippo pathway, TAZ, in the development of aggressive types of endometrial cancer.Hippo 通路转导子 TAZ 在子宫内膜癌侵袭性类型发展中的作用。
Mod Pathol. 2015 Nov;28(11):1492-503. doi: 10.1038/modpathol.2015.102. Epub 2015 Sep 18.
9
Leptin promotes human endometriotic cell migration and invasion by up-regulating MMP-2 through the JAK2/STAT3 signaling pathway.瘦素通过JAK2/STAT3信号通路上调基质金属蛋白酶-2，促进人子宫内膜异位细胞的迁移和侵袭。
Mol Hum Reprod. 2015 Oct;21(10):792-802. doi: 10.1093/molehr/gav039. Epub 2015 Jul 7.
10
PARD3 induces TAZ activation and cell growth by promoting LATS1 and PP1 interaction.PARD3通过促进LATS1与PP1相互作用诱导TAZ激活和细胞生长。
EMBO Rep. 2015 Aug;16(8):975-85. doi: 10.15252/embr.201439951. Epub 2015 Jun 26.