Zheng Y Y, Zhao G
Center for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui, China.
Cryo Letters. 2016 Nov/Dec;37(6):421-426.
BACKGROUND: None-uniform distributions of temperatures, limited freezing and thawing rates, and thermal stresses are three main hindering factors for successful vitreous cryopreservation of mass volume of biosamples. Micro- and nanoscale encapsulation, owning the intrinsic features to avoid those limitations introduced by the traditional approaches, has been opened up a new way for effective and high-efficiency biopreservation.
This short review article summarizes recent advances in cell encapsulation technology for biopreservation by manipulating cells and biological agents in micro- or nanoscale volume droplets and microgels, and discusses its promising applications for future vitreous cryopreservation.
背景：温度分布不均、有限的冻融速率和热应力是生物样本大量体积成功进行玻璃化冷冻保存的三个主要阻碍因素。微米和纳米级封装具有避免传统方法所带来的那些限制的固有特性，为有效且高效的生物保存开辟了一条新途径。
这篇简短的综述文章总结了通过在微米或纳米级体积的液滴和微凝胶中操控细胞和生物制剂进行生物保存的细胞封装技术的最新进展，并讨论了其在未来玻璃化冷冻保存中的潜在应用。
