Chen Po-Cheng, Chiang Kuo-Ping, Tsai Sheng-Fang
Institute of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung, 202-24, Taiwan.
Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, 202-24, Taiwan.
J Eukaryot Microbiol. 2017 Sep;64(5):579-587. doi: 10.1111/jeu.12392. Epub 2017 Feb 10.
The number of somatic kineties in Pelagostrobilidium ranges from 4 to 6 according to the present state of knowledge. This study investigates Pelagostrobilidium liui n. sp. using live observation, protargol stain, and small subunit rDNA data sequencing. Pelagostrobilidium liui n. sp. is characterized by having a spherical-shaped body, four somatic kineties, with kinety 2 spiraled around the left side of body, about six elongated external membranelles, and invariably no buccal membranelle. It differs from its most similar congener, Pelagostrobilidium minutum Liu et al., , in (i) cell shape; (ii) macronucleus width; (iii) oral apparatus; (iv) anterior orientation of kinety 2; (v) location where kinety 2 commences; (vi) arrangement of kinety 1; (vii) distance between the anterior cell end and the locations where kineties commence; and (viii) the presence of 12 different bases (including two deletions) in the small subunit rDNA sequences. The diagnosis of P. minutum Liu et al., is also improved to include the following new characteristics: invariably four somatic kineties; kineties 2 and 4 alone commence at the same level; kinety 2 originates from right anterior cell half on ventral side, extends sinistrally posteriorly, over kinety 1, around left posterior region, terminates near posterior cell end on dorsal side; kinety 1 commences below anterior third of kinety 2.
根据目前的知识状况，Pelagostrobilidium的体动基列数量为4至6个。本研究使用活体观察、原银染法和小亚基核糖体DNA数据测序对刘氏Pelagostrobilidium（新物种）进行了调查。刘氏Pelagostrobilidium（新物种）的特征为具有球形身体、四个体动基列，其中动基列2在身体左侧呈螺旋状，约有六个细长的外动基列膜，且始终没有口动基列膜。它与其最相似的同属物种微小Pelagostrobilidium（Liu等人命名）的区别在于：（i）细胞形状；（ii）大核宽度；（iii）口器；（iv）动基列2的前端方向；（v）动基列2开始的位置；（vi）动基列1的排列；（vii）细胞前端与动基列开始位置之间的距离；以及（viii）小亚基核糖体DNA序列中存在12个不同的碱基（包括两个缺失）。微小Pelagostrobilidium（Liu等人命名）的诊断也得到了改进，包括以下新特征：始终有四个体动基列；只有动基列2和4在同一水平开始；动基列2起源于腹侧细胞右前半部分，向左后方延伸，越过动基列1，围绕左后区域，在背侧细胞后端附近终止；动基列1在动基列2前三分之一下方开始。
