DSouza Roshan, Pinto Naina Adren, Hwang Insik, Younjee Hwang, Cho YoungLag, Kim Hyunsoo, Yong Dongeun, Choi Jongrak, Lee Kyungwon, Chong Yunsop
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.
Brain Korea 21+ Project for Medical Science, Yonsei University, Seoul, Korea Yonsei.
New Microbiol. 2017 Jan;40(1):38-44. Epub 2017 Jan 10.
The aim of this work was to investigate the mechanism responsible for multidrug resistance in ST11 Klebsiella pneumoniae YMC 2013/7/B3993 containing multiple copies of ESBL genes using multiple parallel sequencing technology. In-depth analysis of the strain revealed multiple copies of ESBL genes, 2 copies of blaSHV-12 and 1 copy of blaCTX-M-15. Furthermore, 1 copy of blaOXA-9 and 3 copies of blaTEM-1 were found. The insertion of Tn1331 was detected, which consisted of blaOXA-9, blaTEM-1, aac(6')-lb-cr, and aadA1 genes. The acquisition of multiple copies of resistance genes was due to the insertion of transposons in the bacterial genome and plasmid. The genotypic analysis revealed that the isolates belonging to ST11 showed severe resistance phenotypes and greater dissemination potential. To the best of our knowledge, this is the first report demonstrating multiple copies of same ESBL genes in K. pneumoniae ST11 isolate. Furthermore, massive parallel sequencing studies of genetic factors to enhance the fitness of this type strain would be warranted to determine whether ST11 K. pneumoniae can spread the KPC-type gene.
本研究旨在利用多重平行测序技术，探究携带多个超广谱β-内酰胺酶（ESBL）基因拷贝的ST11型肺炎克雷伯菌YMC 2013/7/B3993的多药耐药机制。对该菌株的深入分析显示存在多个ESBL基因拷贝，包括2个blaSHV-12基因拷贝和1个blaCTX-M-15基因拷贝。此外，还发现了1个blaOXA-9基因拷贝和3个blaTEM-1基因拷贝。检测到Tn1331的插入，其由blaOXA-9、blaTEM-1、aac(6')-lb-cr和aadA1基因组成。耐药基因多个拷贝的获得是由于转座子插入细菌基因组和质粒。基因型分析表明，属于ST11的分离株表现出严重的耐药表型和更大的传播潜力。据我们所知，这是首次报道在肺炎克雷伯菌ST11分离株中存在相同ESBL基因的多个拷贝。此外，有必要对该菌株类型的遗传因素进行大规模平行测序研究，以确定ST11型肺炎克雷伯菌是否能够传播KPC型基因。
