Jeong Chang-Bum, Kim Duck-Hyun, Kang Hye-Min, Lee Young Hwan, Kim Hui-Su, Kim Il-Chan, Lee Jae-Seong
Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, South Korea.
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Aquat Toxicol. 2017 Feb;183:144-155. doi: 10.1016/j.aquatox.2016.12.022. Epub 2016 Dec 30.
The ATP-binding cassette (ABC) protein superfamily is one of the largest gene families and is highly conserved in all domains. The ABC proteins play roles in several biological processes, including multi-xenobiotic resistance (MXR), by functioning as transporters in the cellular membrane. They also mediate the cellular efflux of a wide range of substrates against concentration gradients. In this study, 37 ABC genes belonging to eight distinct subfamilies were identified in the marine copepod Paracyclopina nana and annotated based on a phylogenetic analysis. Also, the functions of P-glycoproteins (P-gp) and multidrug resistance-associated proteins (MRPs), conferring MXR, were verified using fluorescent substrates and specific inhibitors. The activities of MXR-mediated ABC proteins and their transcriptional level were examined in response to polyaromatic hydrocarbons (PAHs), main components of the water-accommodated fraction. This study increases the understanding of the protective role of MXR in response to PAHs over the comparative evolution of ABC gene families.
ATP结合盒（ABC）蛋白超家族是最大的基因家族之一，在所有生物域中高度保守。ABC蛋白在多种生物学过程中发挥作用，包括多异生物质抗性（MXR），其在细胞膜中作为转运蛋白发挥功能。它们还介导多种底物逆浓度梯度的细胞外排。在本研究中，在海洋桡足类动物纳氏伪哲水蚤中鉴定出属于八个不同亚家族的37个ABC基因，并基于系统发育分析进行注释。此外，使用荧光底物和特异性抑制剂验证了赋予MXR的P-糖蛋白（P-gp）和多药耐药相关蛋白（MRP）的功能。针对水相组分的主要成分多环芳烃（PAH），检测了MXR介导的ABC蛋白的活性及其转录水平。本研究通过ABC基因家族的比较进化，增进了对MXR在应对PAH时保护作用的理解。
