Fan Wenchun, Liu Tingting, Li Xiangmin, Zhou Yun, Wu Mengge, Cui Xiaofang, Chen Huanchun, Qian Ping
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Division of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Division of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Mol Immunol. 2017 Feb;82:114-122. doi: 10.1016/j.molimm.2017.01.003. Epub 2017 Jan 7.
Emerging evidence suggests that TRIM family proteins play a crucial role in regulating the NF-κB signaling pathway. TRIM52 is a novel noncanonical antiviral TRIM gene with a unique expanded RING domain. Information on the biological function of TRIM52 is limited. Herein, we demonstrated TRIM52 involvement in NF-κB activation. We found that TRIM52 overexpression specifically activated the NF-κB signal. TRIM52 overexpression can significantly induce TNFα and IL-6 expression. We also found that the RING domain of TRIM52 was essential for its activation of the NF-κB signal. Further study showed that TRIM52 overexpression did not affect the protein level of IκBα and phosphorylated p65 protein. We found that the pro-inflammatory cytokines TNFα and IL-6 could induce TRIM52 expression. Overall, these data suggested that TRIM52 was a positive regulator of the NF-κB pathway.
新出现的证据表明，TRIM家族蛋白在调节核因子-κB（NF-κB）信号通路中起关键作用。TRIM52是一种新型的非典型抗病毒TRIM基因，具有独特的扩展型RING结构域。关于TRIM52生物学功能的信息有限。在此，我们证明了TRIM52参与NF-κB激活。我们发现TRIM52过表达特异性激活NF-κB信号。TRIM52过表达可显著诱导肿瘤坏死因子α（TNFα）和白细胞介素-6（IL-6）表达。我们还发现TRIM52的RING结构域对其激活NF-κB信号至关重要。进一步研究表明，TRIM52过表达不影响IκBα蛋白水平和磷酸化p65蛋白。我们发现促炎细胞因子TNFα和IL-6可诱导TRIM52表达。总体而言，这些数据表明TRIM52是NF-κB通路的正调节因子。
