TRIM52：一种正向调节核因子κB信号通路的核内TRIM蛋白。

TRIM52: A nuclear TRIM protein that positively regulates the nuclear factor-kappa B signaling pathway.

作者信息

Fan Wenchun, Liu Tingting, Li Xiangmin, Zhou Yun, Wu Mengge, Cui Xiaofang, Chen Huanchun, Qian Ping

机构信息

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Division of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Mol Immunol. 2017 Feb;82:114-122. doi: 10.1016/j.molimm.2017.01.003. Epub 2017 Jan 7.

DOI:10.1016/j.molimm.2017.01.003

PMID:28073078

Abstract

Emerging evidence suggests that TRIM family proteins play a crucial role in regulating the NF-κB signaling pathway. TRIM52 is a novel noncanonical antiviral TRIM gene with a unique expanded RING domain. Information on the biological function of TRIM52 is limited. Herein, we demonstrated TRIM52 involvement in NF-κB activation. We found that TRIM52 overexpression specifically activated the NF-κB signal. TRIM52 overexpression can significantly induce TNFα and IL-6 expression. We also found that the RING domain of TRIM52 was essential for its activation of the NF-κB signal. Further study showed that TRIM52 overexpression did not affect the protein level of IκBα and phosphorylated p65 protein. We found that the pro-inflammatory cytokines TNFα and IL-6 could induce TRIM52 expression. Overall, these data suggested that TRIM52 was a positive regulator of the NF-κB pathway.

摘要

新出现的证据表明，TRIM家族蛋白在调节核因子-κB（NF-κB）信号通路中起关键作用。TRIM52是一种新型的非典型抗病毒TRIM基因，具有独特的扩展型RING结构域。关于TRIM52生物学功能的信息有限。在此，我们证明了TRIM52参与NF-κB激活。我们发现TRIM52过表达特异性激活NF-κB信号。TRIM52过表达可显著诱导肿瘤坏死因子α（TNFα）和白细胞介素-6（IL-6）表达。我们还发现TRIM52的RING结构域对其激活NF-κB信号至关重要。进一步研究表明，TRIM52过表达不影响IκBα蛋白水平和磷酸化p65蛋白。我们发现促炎细胞因子TNFα和IL-6可诱导TRIM52表达。总体而言，这些数据表明TRIM52是NF-κB通路的正调节因子。