Jansson B, Brodin T, Sjögren H O
Dept. of Tumour Immunology, University of Lund, Sweden.
Hybridoma. 1989 Oct;8(5):493-505. doi: 10.1089/hyb.1989.8.493.
In this report we describe an auto-immunogenic tumor associated high molecular weight glycoprotein (HMW-GP) present on most rat colon carcinomas as detected by syngeneic monoclonal IgM antibodies. The HMW-GP has an apparent molecular weight of more than 10(6) D and carries several epitopes for various lectins, carbohydrate specific mouse monoclonal antibodies and four rat syngeneic monoclonal antibodies. The epitope defined by the 10B 12 rat monoclonal antibody is present in colon carcinoma tissue, but only in very low amounts in normal gastro-intestinal-tissue. The epitope is present at multiple sites on the large molecule and is shown to be sterically related, but not identical to determinants for Dolichos biflorus lectin and a mouse mAB binding to blood group A. The 10B 12 mAB binding is insensitive to boiling, reduction with mercaptoethanol and treatment with trypsin, but abolished by pretreatment of the antigen with sodium periodate or neuraminidase. The epitopes defined by 1 G6 and 1F6 rat monoclonal antibodies are present both in tumor tissue and in normal colon mucosa and the antibody binding is increased after treatment with both neuraminidase and sodium periodate.
在本报告中，我们描述了一种自身免疫原性肿瘤相关的高分子量糖蛋白（HMW - GP），它存在于大多数大鼠结肠癌中，可通过同基因单克隆IgM抗体检测到。该HMW - GP的表观分子量超过10⁶D，带有多种凝集素、碳水化合物特异性小鼠单克隆抗体和四种大鼠同基因单克隆抗体的多个表位。由10B 12大鼠单克隆抗体定义的表位存在于结肠癌组织中，但在正常胃肠道组织中的含量极低。该表位存在于大分子的多个位点，并且显示出空间相关性，但与双花扁豆凝集素和一种结合A血型的小鼠单克隆抗体的决定簇不同。10B 12单克隆抗体的结合对煮沸、用巯基乙醇还原和用胰蛋白酶处理不敏感，但在用高碘酸钠或神经氨酸酶预处理抗原后被消除。由1G6和1F6大鼠单克隆抗体定义的表位既存在于肿瘤组织中，也存在于正常结肠黏膜中，并且在用神经氨酸酶和高碘酸钠处理后抗体结合增加。
