Genne P, Olsson N O, Caignard A, Oriol R, Bara J, Hammann A, Martin F
Research Group on Digestive Tumours, INSERM U252, Faculty of Medecine, Dijon, France.
Virchows Arch A Pathol Anat Histopathol. 1992;420(3):233-42. doi: 10.1007/BF01600276.
A monoclonal antibody, F11C, was raised against rat colon cancer cells. Its immunoreactivity on normal human and rat gut as well as human and rat colonic tumours was studied by the avidin-biotin-peroxidase complex technique. In both normal rat and human gastrointestinal tract, F11C stained surface epithelial cells from the fundus to distal colon, mainly as supranuclear vesicles. These vesicles appeared to be part of the Golgi apparatus on electron microscopy with immunogold labelling. Twenty primary rat colon tumours and 28 of 43 human colon tumours were also stained, with a heterogeneous pattern but much more strongly than the normal colonic mucosa. Biochemical purification suggested that in rat tumours F11C epitope was carried by a high molecular weight glycoprotein. Absorption experiments with synthetic oligosaccharides showed that F11C monoclonal antibody reacted with blood group A-related oligosaccharides. Nevertheless, F11C reactivity on human tissues was not related to the individual ABO or Lewis phenotype.
制备了一种针对大鼠结肠癌细胞的单克隆抗体F11C。采用抗生物素蛋白-生物素-过氧化物酶复合物技术研究了其在正常人和大鼠肠道以及人和大鼠结肠肿瘤上的免疫反应性。在正常大鼠和人的胃肠道中，F11C对从胃底到结肠远端的表面上皮细胞进行染色，主要表现为核上小泡。通过免疫金标记的电子显微镜观察，这些小泡似乎是高尔基体的一部分。20例原发性大鼠结肠肿瘤和43例人类结肠肿瘤中的28例也被染色，呈现出异质性模式，但比正常结肠黏膜染色更强。生化纯化表明，在大鼠肿瘤中，F11C表位由一种高分子量糖蛋白携带。用合成寡糖进行的吸收实验表明，F11C单克隆抗体与A血型相关寡糖发生反应。然而，F11C在人体组织上的反应性与个体的ABO或Lewis血型表型无关。
