Jorge Antônio Sérgio Barcala, Jorge Gislaine Candida Batista, Paraíso Alanna Fernandes, Franco Raíssa M Porto, Vieira Lara Jhullian Tolentino, Hilzenderger Aline Mourão, Guimarães André Luiz Sena, Andrade João Marcus Oliveira, De-Paula Alfredo M Batista, Santos Sergio Henrique S
Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
Medical School. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
Exp Clin Endocrinol Diabetes. 2017 Mar;125(3):163-170. doi: 10.1055/s-0042-119525. Epub 2017 Jan 10.
The present study aimed to analyze the expression of IL6, UCP1 and SIRT1 in adipose tissue (WAT and BAT) in association to clinical, metabolic and anthropometric parameters in obese humans. WAT and BAT samples from obese patients (n=27) were collected. IL6, UCP1 and SIRT1 markers were measured by qRT-PCR. The association between IL6, UCP1 and SIRT1 mRNA expression and anthropometric and clinical parameters were evaluated, using appropriate statistical tests. Our results demonstrated that high levels of IL6 are associated with altered glucose levels in the WAT (p=0.01). In contrast, high levels of IL6 in the BAT were associated with decreased % fat (p=0.01) and fat weight (p=0.02) and increased mVO (p=0.02) and VO (p=0.02). For UCP1, a higher expression in the BAT was observed when compared to the WAT (p=0.0001). This gene expression was associated with lower values of BMI (p=0.03), % fat (P=0.02) and fat weight (P=0.02) and increased mVO (p=0.041) and VO (p=0.001). In the WAT, decreased levels of SIRT1 were associated with increased fat weight (p=0.02); in the BAT, associations were found for % fat (p=0.018) and mVO (p=0.03). These results reveal different characteristics in the biological actions between WAT and BAT in obese humans. Increased levels of IL6, UCP1 and SIRT1 in the BAT were associated with metabolic parameters improvements.
本研究旨在分析肥胖人群脂肪组织（白色脂肪组织和棕色脂肪组织）中白细胞介素6（IL6）、解偶联蛋白1（UCP1）和沉默信息调节因子1（SIRT1）的表达，并将其与临床、代谢和人体测量学参数相关联。收集了27例肥胖患者的白色脂肪组织和棕色脂肪组织样本。通过定量逆转录聚合酶链反应（qRT-PCR）检测IL6、UCP1和SIRT1标志物。使用适当的统计检验评估IL6、UCP1和SIRT1信使核糖核酸（mRNA）表达与人体测量学和临床参数之间的关联。我们的结果表明，白色脂肪组织中高水平的IL6与血糖水平改变相关（p=0.01）。相反，棕色脂肪组织中高水平的IL6与体脂百分比降低（p=0.01）、脂肪重量降低（p=0.02）以及静息代谢率增加（p=0.02）和代谢率增加（p=0.02）相关。对于UCP1，与白色脂肪组织相比，棕色脂肪组织中的表达更高（p=0.0001）。这种基因表达与较低的体重指数值（p=0.03）、体脂百分比（P=0.02）和脂肪重量（P=0.02）以及静息代谢率增加（p=0.041）和代谢率增加（p=0.001）相关。在白色脂肪组织中，SIRT1水平降低与脂肪重量增加相关（p=0.02）；在棕色脂肪组织中，发现与体脂百分比（p=0.018）和静息代谢率（p=0.03）有关联。这些结果揭示了肥胖人群白色脂肪组织和棕色脂肪组织生物学作用的不同特征。棕色脂肪组织中IL6、UCP1和SIRT1水平升高与代谢参数改善相关。
