Department of Biology, Kongju National University, Kongju 314-701, Republic of Korea.
Department of Biology, Kongju National University, Kongju 314-701, Republic of Korea.
Harmful Algae. 2016 Feb;52:23-33. doi: 10.1016/j.hal.2015.12.004. Epub 2015 Dec 29.
Photosynthesis in the ciliate Mesodinium rubrum is achieved using a consortium of cryptophyte algal organelles enclosed in its specialized vacuole. A time-series microarray analysis was conducted on the photosynthetic ciliate using an oligochip containing 15,654 primers designed from EST data of the cryptophyte prey, Teleaulax amphioxeia. The cryptophycean nuclei were transcriptionally active over 13 weeks and approximately 13.5% of transcripts in the ciliate came from the sequestered nuclei. The cryptophyte nuclei and chloroplasts could divide in the ciliate, which were loosely synchronized with host cell division. A large epigenetic modification occurred after the cryptophyte nuclei were sequestered into the ciliate. Most cryptophyte genes involved in the light and dark reactions of photosynthesis, chlorophyll assimilation, as well as in DNA methylation, were consistently up-regulated in the ciliate. The imbalance of division rate between the sequestered cryptophyte nuclei and host nuclei may be the reason for the eventual cessation of the kleptoplastidy.
纤毛虫 Mesodinium rubrum 的光合作用是通过其专门的液泡中包含的隐藻藻类细胞器的联合体来实现的。使用包含 15654 个引物的寡核苷酸芯片对含有隐藻猎物 Teleaulax amphioxeia 的 EST 数据进行了时间序列微阵列分析。隐藻核在 13 周内具有转录活性，纤毛虫中约有 13.5%的转录本来自被隔离的核。隐藻核和叶绿体可以在纤毛虫中分裂，与宿主细胞分裂大致同步。在隐藻核被隔离到纤毛虫中后，发生了大规模的表观遗传修饰。光合作用的光和暗反应、叶绿素同化以及 DNA 甲基化中涉及的大多数隐藻基因在纤毛虫中持续上调。被隔离的隐藻核与宿主核之间分裂率的不平衡可能是最终停止偷叶绿体的原因。
