An Jian-Ping, Li Rui, Qu Feng-Jia, You Chun-Xiang, Wang Xiao-Fei, Hao Yu-Jin
National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.
National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.
Biochem Biophys Res Commun. 2017 Jan 29;483(1):1-9. doi: 10.1016/j.bbrc.2017.01.026. Epub 2017 Jan 7.
Cytochrome P450s play an important role in plant growth and are involved in multiple stresses response. However, little is known about the functions of cytochrome P450s in apple. Here, a Malus × domestica cytochrome P450 monooxygenase 1 gene, MdCYPM1, was identified and subsequently cloned from apple 'Gala' (Malus × domestica). To verify the functions of MdCYPM1, we generated transgenic Arabidopsis plants expressing the apple MdCYPM1 gene under the control of the Cauliflower mosaic virus 35S promoter. Four transgenic lines (#3, #5, #7 and #8) were selected for further study. The transgenic plants exhibited a series of skotomorphogenesis phenotypes relative to wild-type controls, such as reduction of the chlorophyll, anthocyanins content and hypocotyls elongation. In addition, overexpression of MdCYPM1 influenced auxin transport and flowering time in transgenic Arabidopsis. Furthermore, MdCYPM1 expression was induced by salt and mannitol treatments, and the transgenic plants were negatively regulated by salinity and osmotic stresses during germination. These results suggest that MdCYPM1 plays a vital role in plant growth and development.
细胞色素P450在植物生长中发挥着重要作用，并参与多种胁迫反应。然而，关于细胞色素P450在苹果中的功能却知之甚少。在此，我们从苹果‘嘎啦’（Malus × domestica）中鉴定并克隆了一个苹果细胞色素P450单加氧酶1基因，MdCYPM1。为了验证MdCYPM1的功能，我们构建了在花椰菜花叶病毒35S启动子控制下表达苹果MdCYPM1基因的转基因拟南芥植株。挑选了4个转基因株系（#3、#5、#7和#8）进行进一步研究。相对于野生型对照，转基因植株表现出一系列暗形态建成表型，如叶绿素含量、花青素含量降低以及下胚轴伸长受抑制。此外，MdCYPM1的过表达影响了转基因拟南芥中的生长素运输和开花时间。此外，盐处理和甘露醇处理可诱导MdCYPM1表达，并且转基因植株在萌发过程中受到盐胁迫和渗透胁迫的负调控。这些结果表明，MdCYPM1在植物生长发育中起着至关重要的作用。
