College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing 210037, China.
Genes (Basel). 2021 May 17;12(5):754. doi: 10.3390/genes12050754.
We evaluated a transcriptome using high-throughput Illumina HiSeq sequencing and related it to the morphology, leaf anatomy, and physiological parameters of under NO stress. The molecular mechanism of the NO stress response was evaluated using sequencing data. NO stress adversely affected the morphology, leaf anatomy, and total peroxidase (POD) activity. Through RNA-seq analysis, we used NCBI to compare the transcripts with nine databases and obtained their functional annotations. We annotated up to 2255 million clean Illumina paired-end RNA-seq reads, and 250,200 unigene sequences were assembled based on the resulting transcriptome data. More than 89% of the transcripts were functionally annotated. Under NO stress, 1119 genes were upregulated and 1240 were downregulated. According to the KEGG pathway and GO analyses, photosynthesis, chloroplasts, plastids, and the stimulus response are related to NO stress. Additionally, NO stress changed the expression of POD families, and the , , and genes exhibited high expression. The transcriptome analysis of leaves under NO stress supplies a reference for studying the molecular mechanism of resistance to NO stress. The given transcriptome data represent a valuable resource for studies on plant genes, which will contribute towards genome annotations during future genome projects.
我们使用高通量 Illumina HiSeq 测序评估了转录组，并将其与 在无氮胁迫下的形态、叶片解剖结构和生理参数相关联。使用测序数据评估了 对氮胁迫的分子机制。氮胁迫对形态、叶片解剖结构和总过氧化物酶（POD）活性产生不利影响。通过 RNA-seq 分析，我们使用 NCBI 将转录本与九个数据库进行比较，并获得了它们的功能注释。我们对多达 2.255 亿个干净的 Illumina 配对末端 RNA-seq reads 进行了注释，并根据所得转录组数据组装了 250200 个 unigene 序列。超过 89%的 转录本被功能注释。在氮胁迫下，有 1119 个基因上调，1240 个基因下调。根据 KEGG 通路和 GO 分析，光合作用、叶绿体、质体和刺激反应与氮胁迫有关。此外，氮胁迫改变了 POD 家族的表达， 、 和 基因表现出高表达。氮胁迫下 叶片的转录组分析为研究 对氮胁迫的抗性分子机制提供了参考。所提供的转录组数据为植物基因研究提供了有价值的资源，这将有助于未来基因组计划中的基因组注释。
