Analytical performances of Optilite turbidimeter (The Binding Site): a new dedicated analyser for specific proteins determination.

We checked analytical performances of Optilite analyser for immunoglobulins G, A, M, subclasses of IgG, free light chains of Ig (Freelite) and complement's fractions using Binding Site reagents. CVs for repeteability and reproducibility showed very good results, respectively <3% and <10% for all tested parameters, in agreement with Ricos and SFBC recommendations. Comparisons with results obtained on BNII (Siemens) or SPAPLUS ® (Binding site) analysers showed a good agreement (>83%) according to Bland and Altman analysis. Sample throughput with either a batch of Freelite only or Freelite and immunoglobulins showed a gain of total realisation time on Optilite versus BNII. Optilite analyser performed automatic dilutions until result and antigen excess determination for parameters as Freelite or IgG4. In terms of practicability, traceability and maintenance, Optilite turbidimeter alone or connected to LIS via DataSite software is well adapted to specialized laboratory for proteins determinations.