Abdul Shukkoor Mohamed Saleem, Baharuldin Mohamad Taufik Hidayat Bin, Mat Jais Abdul Manan, Mohamad Moklas Mohamad Aris, Fakurazi Sharida
Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
Evid Based Complement Alternat Med. 2016;2016:2986090. doi: 10.1155/2016/2986090. Epub 2016 Dec 18.
This study evaluated the antidepressant-like effect of lipid extract of in chronic unpredictable mild stress (CUMS) model of depression in male rats and its mechanism of action. The animals were subjected to CUMS for six weeks by using variety of stressors. At the end of CUMS protocol, animals were subjected to forced swimming test (FST) and open field test followed by biochemical assay. The CUMS protocol produced depressive-like behavior in rats by decreasing the body weight, decreasing the sucrose preference, and increasing the duration of immobility in FST. The CUMS protocol increased plasma corticosterone and decreased hippocampal and prefrontal cortex levels of monoamines (serotonin, noradrenaline, and dopamine) and brain-derived neurotrophic factor. Further, the CUMS protocol increased interleukin-6 (in hippocampus and prefrontal cortex) and nuclear factor-kappa B (in prefrontal cortex but not in hippocampus). The lipid extract of (125, 250, and 500 mg/kg) significantly ( < 0.05) reversed all the above parameters in rats subjected to CUMS, thus exhibiting antidepressant-like effect. The mechanism was found to be mediated through decrease in plasma corticosterone, increase in serotonin levels in prefrontal cortex, increase in dopamine and noradrenaline levels in hippocampus and prefrontal cortex, increase in BDNF in hippocampus and prefrontal cortex, and decrease in IL-6 and NF-B in prefrontal cortex.
本研究评估了[具体物质]脂质提取物在雄性大鼠慢性不可预测轻度应激（CUMS）抑郁模型中的抗抑郁样作用及其作用机制。通过使用多种应激源使动物经受六周的CUMS。在CUMS实验方案结束时，对动物进行强迫游泳试验（FST）和旷场试验，随后进行生化分析。CUMS实验方案通过降低体重、降低蔗糖偏好以及增加FST中的不动时间，在大鼠中产生了抑郁样行为。CUMS实验方案增加了血浆皮质酮水平，并降低了海马体和前额叶皮质中单胺类物质（血清素、去甲肾上腺素和多巴胺）以及脑源性神经营养因子的水平。此外，CUMS实验方案增加了白细胞介素-6（在海马体和前额叶皮质中）以及核因子-κB（在前额叶皮质中而非海马体中）。[具体物质]脂质提取物（125、250和500mg/kg）显著（P<0.05）逆转了经受CUMS大鼠的上述所有参数，从而表现出抗抑郁样作用。发现其作用机制是通过降低血浆皮质酮水平、增加前额叶皮质中的血清素水平、增加海马体和前额叶皮质中的多巴胺和去甲肾上腺素水平、增加海马体和前额叶皮质中的脑源性神经营养因子以及降低前额叶皮质中的白细胞介素-6和核因子-κB来介导的。
