多壁碳纳米管的致突变性缺失：使用F344 δ大鼠的单次气管内滴注研究
Absence of mutagenicity of multi-walled carbon nanotubes single intratracheal instillation study using F344 delta rats.
作者信息
Horibata Katsuyoshi, Ukai Akiko, Ogata Akio, Nakae Dai, Ando Hiroshi, Kubo Yoshikazu, Nagasawa Akemichi, Yuzawa Katsuhiro, Honma Masamitsu
机构信息
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo, 158-8501 Japan.
Department of Pharmaceutical and Environmental Sciences, Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunin-cho, Shinjuku, Tokyo, 169-0073 Japan.
出版信息
Genes Environ. 2017 Jan 6;39:4. doi: 10.1186/s41021-016-0065-5. eCollection 2017.
INTRODUCTION
It is known that fibrous particles of micrometer length, such as carbon nanotubes, which have same dimensions as asbestos, are carcinogenic. Carcinogenicity of nanomaterials is strongly related to inflammatory reactions; however, the genotoxicity mechanism(s) is unclear. Indeed, inconsistent results on genotoxicity of multi-walled carbon nanotubes (MWCNTs) have been shown in several reports. Therefore, we analyzed the in vivo genotoxicity induced by an intratracheal instillation of straight MWCNTs in rats using a different test system-the gene mutation assay-that can reflect the genotoxicity occurring in the bone marrow. Since lungs were directly exposed to MWCNTs upon intratracheal instillation, we also performed the assay using the lungs.
FINDINGS
We detected no significant differences in mutant frequencies (MFs) between the MWCNT-treated and control rats. Additionally, we detected no significant differences in MFs in the lung between the MWCNT-treated and control rats.
CONCLUSIONS
Our findings indicated that a single intratracheal instillation of MWCNTs was non-mutagenic to both the bone marrow and lung of rats.
引言
众所周知，微米级长度的纤维状颗粒，如与石棉尺寸相同的碳纳米管，具有致癌性。纳米材料的致癌性与炎症反应密切相关；然而，其基因毒性机制尚不清楚。事实上，几份报告显示多壁碳纳米管（MWCNT）的基因毒性结果并不一致。因此，我们使用一种不同的测试系统——基因突变试验，分析了经气管内注入直链MWCNT诱导的大鼠体内基因毒性，该试验能够反映骨髓中发生的基因毒性。由于经气管内注入后肺部直接接触MWCNT，我们还使用肺部进行了该试验。
研究结果
我们在MWCNT处理组和对照组大鼠之间未检测到突变频率（MF）的显著差异。此外，我们在MWCNT处理组和对照组大鼠的肺部MF中也未检测到显著差异。
结论
我们的研究结果表明，单次经气管内注入MWCNT对大鼠的骨髓和肺部均无致突变性。
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