母猪向其后代转移β-羟基-β-甲基丁酸及其对猪肌肉纤维类型转化和性能的影响。

Transfer of -hydroxy--methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs.

作者信息

Wan Haifeng, Zhu Jiatao, Wu Caimei, Zhou Pan, Shen Yong, Lin Yan, Xu Shengyu, Che Lianqiang, Feng Bin, Li Jian, Fang Zhengfeng, Wu De

机构信息

Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu, 611130 Sichuan People's Republic of China.

出版信息

J Anim Sci Biotechnol. 2017 Jan 7;8:2. doi: 10.1186/s40104-016-0132-6. eCollection 2017.

BACKGROUND

Previous studies suggested that supplementation of lactating sows with -hydroxy--methylbutyrate (HMB) could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and 180 after birth. A total of 20 sows according to their body weight were divided into the control (CON, = 10) or HMB groups (HMB, = 10). Sows in the HMB group were supplemented with -hydroxy--methylbutyrate calcium (HMB-Ca) 2 g /kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28 ( = 6/treatment) and 180 ( = 6/treatment) postnatal, and the longissimus dorsi (LD) was collected, respectively.

RESULTS

The HMB-fed sows during lactation showed increased HMB concentration ( < 0.05) in milk and LD of weaning piglets ( < 0.05). In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group ( < 0.05), meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type II muscle fiber cross-sectional area (CSA), elevated myosin heavy chain (MyHC) and mRNA, and fast-MyHC protein levels in LD ( < 0.05).

CONCLUSIONS

HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation.

背景

先前的研究表明，给哺乳母猪补充β-羟基-β-甲基丁酸（HMB）可提高断奶仔猪的性能，但关于母体摄入HMB后其后代肌肉纤维类型转变以及断奶至育肥阶段后续生长性能的信息较少。本研究的目的是确定给哺乳母猪补充HMB对出生后28天和180天后代骨骼肌纤维类型转变和生长的影响。根据体重将20头母猪分为对照组（CON，n = 10）或HMB组（HMB，n = 10）。HMB组母猪在哺乳期第1至27天，每千克饲料中补充2克β-羟基-β-甲基丁酸钙（HMB-Ca）。断奶后，48头混合性别的仔猪按母猪处理方式进行分组，并在断奶、生长、育肥期饲喂标准日粮。记录出生后28至180天的生长性能。分别在出生后28天（每组6头）和180天（每组6头）屠宰猪，采集背最长肌（LD）。

结果

哺乳期饲喂HMB的母猪所产断奶仔猪的乳汁和LD中HMB浓度升高（P < 0.05）。此外，HMB组后代的育肥体重和瘦肉率高于CON组猪（P < 0.05），同时，与饲喂CON日粮母猪所产仔猪相比，饲喂HMB日粮母猪所产仔猪的LD中II型肌纤维横截面积（CSA）更大，肌球蛋白重链（MyHC）IIx和IIb mRNA水平升高，快肌型MyHC蛋白水平更高（P < 0.05）。