Aydın Arif, Yıldırım Alpagan Mustafa
Department of Plastic Reconstructive and Aesthetic Surgery, Afyonkarahisar State Hospital, Afyonkarahisar-Turkey.
Ulus Travma Acil Cerrahi Derg. 2016 Nov;22(6):509-515. doi: 10.5505/tjtes.2016.00258.
This study was performed to evaluate the effect of alpha lipoic acid (ALA) on prevention of ischemia-reperfusion (IR) injury in rat hindlimb ischemia model.
Forty male Sprague Dawley rats weighing between 250 and 300 g were divided into 4 groups of 10 rats. Hindlimb composite island flaps were raised in all rats. Clamps were applied to femoral vessels of all subjects, but immediately released without causing ischemia in Group 1. In Group 2, after 4 hours of ischemia, 24 hours of reperfusion was performed. Following 4 hours of ischemia, saline was administered to rats in Group 3 and flaps were reperfused for 24 hours. In Group 4, ALA was administered intraperitoneally after 4 hours of ischemia and flaps were reperfused for 24 hours.
In Group 4, there was a significant decrease of liver malondialdehyde compared to Group 2 and decrease of muscle tumor necrosis factor-alpha compared to Group 3. There was also increase in levels of glutathione in erythrocytes compared to Group 3 and increase of plasma vitamin C compared to all groups.
ALA was found to be effective in prevention of ischemia-reperfusion injury. Further studies are needed before clinical application.
本研究旨在评估α硫辛酸（ALA）对大鼠后肢缺血模型中缺血再灌注（IR）损伤的预防作用。
将40只体重在250至300克之间的雄性Sprague Dawley大鼠分为4组，每组10只。所有大鼠均制备后肢复合岛状皮瓣。对所有受试动物的股血管施加血管夹，但第1组在施加后立即松开，不造成缺血。第2组在缺血4小时后进行24小时再灌注。第3组在缺血4小时后给大鼠注射生理盐水，皮瓣再灌注24小时。第4组在缺血4小时后腹腔注射ALA，皮瓣再灌注24小时。
与第2组相比，第4组肝脏丙二醛水平显著降低；与第3组相比，第4组肌肉肿瘤坏死因子-α水平降低。与第3组相比，第4组红细胞中谷胱甘肽水平升高，与所有组相比血浆维生素C水平升高。
发现ALA对预防缺血再灌注损伤有效。在临床应用前还需要进一步研究。
