Wang Huili, Chen Zhijiang, Chang Hongen, Mu Xiaoping, Deng Wenyu, Yuan Zhaohu, Yao Fang, Liu Yan, Mai Rongjia, Wu Bingyi
Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000, P.R. China.
Pediatric Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510000, P.R. China.
Oncol Rep. 2017 Feb;37(2):929-936. doi: 10.3892/or.2017.5361. Epub 2017 Jan 9.
Glia maturation factor γ (GMFG) functions to reorganize the actin cytoskeleton and appears to play a causative role in cell migration and adherence. The present study assessed GMFG expression in colorectal cancer cells and tissue specimens and then explored the role of GMFG in colorectal cancer progression in vitro. GMFG protein was highly expressed in colorectal cancer tissues and a metastatic colon cancer cell line. Knockdown of GMFG expression using GMFG siRNA or anti-GMFG antibody decreased the capacity of colon cancer LoVo cell migration and invasion in vitro, while recombinant GMFG treatment induced LoVo cell migration. Furthermore, GMFG knockdown also decreased expression of MMP2 protein and reversed epithelial-mesenchymal transition (EMT) phenotypes in LoVo cells. Co-culture of LoVo cells with human umbilical vein endothelial cells (HUVECs) and exogenous GMFG treatment promoted LoVo cell migration and invasion. The data from the present study indicate that GMFG should be further evaluated as a biomarker for detection of colorectal cancer metastasis and that the targeting of GMFG expression or function could be a novel strategy in the future control of colorectal cancer.
神经胶质成熟因子γ（GMFG）的作用是重组肌动蛋白细胞骨架，并且似乎在细胞迁移和黏附中发挥着重要作用。本研究评估了GMFG在大肠癌细胞和组织标本中的表达情况，然后在体外探究了GMFG在大肠癌进展中的作用。GMFG蛋白在大肠癌组织和一种转移性结肠癌细胞系中高表达。使用GMFG小干扰RNA（siRNA）或抗GMFG抗体敲低GMFG表达，可降低体外培养的结肠癌LoVo细胞的迁移和侵袭能力，而重组GMFG处理则诱导LoVo细胞迁移。此外，敲低GMFG还降低了MMP2蛋白的表达，并逆转了LoVo细胞中的上皮-间质转化（EMT）表型。将LoVo细胞与人脐静脉内皮细胞（HUVECs）共培养以及外源性GMFG处理可促进LoVo细胞的迁移和侵袭。本研究的数据表明，GMFG应作为检测大肠癌转移的生物标志物进行进一步评估，并且靶向GMFG的表达或功能可能是未来控制大肠癌的一种新策略。
