Moog C, Luu B, Altmeyer A, Bischoff P
Laboratoire de Chimie Organique des Substances Naturelles, UA 31, CNRS, Strasbourg, France.
Int J Immunopharmacol. 1989;11(5):559-65. doi: 10.1016/0192-0561(89)90186-0.
The effects of an oxysterol, 7,25-dihydroxycholesterol (7,25-OHC), cyclosporin A (CsA) and dexamethasone (Dex) on the blastogenic response of murine lymphocytes to various stimuli were investigated. 7,25-OHC markedly depressed the response to Con A, anti-T3 monoclonal antibodies and to the combination phorbol myristate acetate (PMA) + IL-2 and PMA + ionomycin. Dexamethasone was also active within the same range of concentrations. However, it did not inhibit the stimulation induced by PMA + ionomycin. On the other hand, Cs A failed to depress the lymphocyte response to IL-2 + PMA. Therefore, the mechanisms of action involved in the blockage of lymphocyte activation are different for these three compounds. Moreover, we noted that 7,25-OHC was less active upon purified T-cells. Taken together, these results suggest that 7,25-OHC may act at the level of signal transduction across the membrane.
研究了一种氧化甾醇7,25-二羟基胆固醇（7,25-OHC）、环孢菌素A（CsA）和地塞米松（Dex）对小鼠淋巴细胞对各种刺激的增殖反应的影响。7,25-OHC显著抑制了对刀豆球蛋白A、抗T3单克隆抗体以及佛波醇肉豆蔻酸酯乙酸盐（PMA）+白细胞介素-2和PMA+离子霉素组合的反应。地塞米松在相同浓度范围内也有活性。然而，它并不抑制PMA+离子霉素诱导的刺激。另一方面，CsA未能抑制淋巴细胞对IL-2+PMA的反应。因此，这三种化合物在阻断淋巴细胞活化中所涉及的作用机制不同。此外，我们注意到7,25-OHC对纯化的T细胞活性较低。综上所述，这些结果表明7,25-OHC可能作用于跨膜信号转导水平。
