del Gobbo V, Libri V, Villani N, Caliò R, Nisticò G
Department of Experimental Medicine, II University of Rome, Italy.
Int J Immunopharmacol. 1989;11(5):567-73. doi: 10.1016/0192-0561(89)90187-2.
Four--five-week-old C57BL/6 mice were surgically pinealectomized. At different time intervals after surgery their spleens were removed and assayed for interleukin-2 (IL-2) production and natural killer (NK) cell activity. Non-operated and sham-operated mice were used as controls. The present results indicate that pinealectomy significantly reduced IL-2 production and NK cell activity, in comparison to sham-operated mice. These effects seem to be related to the lack of melatonin. In fact the subcutaneous injection of this hormone (50 or 100 mg/kg at 5 p.m.) in pinealectomized mice was able to restore IL-2 production and NK cell activity. However, chronic treatment with melatonin (10, 20 and 50 mg/kg for 9 consecutive days) failed to reverse the impairment of the immune responses.
对4至5周龄的C57BL/6小鼠进行松果体切除手术。在手术后的不同时间间隔，切除它们的脾脏并检测白细胞介素-2（IL-2）的产生和自然杀伤（NK）细胞活性。未手术和假手术的小鼠用作对照。目前的结果表明，与假手术小鼠相比，松果体切除显著降低了IL-2的产生和NK细胞活性。这些影响似乎与褪黑素缺乏有关。事实上，对松果体切除的小鼠皮下注射这种激素（下午5点注射50或100毫克/千克）能够恢复IL-2的产生和NK细胞活性。然而，褪黑素的长期治疗（连续9天给予10、20和50毫克/千克）未能逆转免疫反应的损伤。
