Lins Rodrigo Rego, Oshima Celina Tizuko Fujiyama, Oliveira Levindo Alves de, Silva Marcelo Souza, Mader Ana Maria Amaral Antonio, Waisberg Jaques
Postgraduate Program in Interdisciplinary Surgical Science, Federal University of São Paulo - UNIFESP, São Paulo, SP; Brazil.
Department of Pathology, UNIFESP, São Paulo, SP, Brazil.
Arq Bras Cir Dig. 2016 Nov-Dec;29(4):227-231. doi: 10.1590/0102-6720201600040004.
Gastric cancer is the fifth most frequent cancer and the third most common cause of cancer-related deaths worldwide.It has been reported that Wnt/ betacatenin pathway is activated in 30-50% of these tumors. However,the deregulation of this pathway has not been fully elucidated.
To determine the expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins in gastric adenocarcinoma tissues and correlate with clinical and pathological parameters.
Seventy-one patients with gastric adenocarcinoma undergoing gastrectomy were enrolled. The expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins was detected by immunohistochemistryand related to the clinical and pathological parameters.
The expression rates of E-cadherin in the membrane was 3%; betacatenin in the cytoplasm and nucleus were 23,4% and 3,1% respectively; APC in the cytoplasm was 94,6%; TCF-4 in the nucleus was 19,4%; and survivin in the nucleus 93,9%. The expression rate of E-cadherin was correlated with older patients (p=0,007), while betacatenin with tumors <5 cm (p=0,041) and APC with proximal tumors (p=0,047). Moreover, the expression of TCF-4 was significantly higher in the diffuse type (p=0,017) and T4 tumors (p=0,002).
The Wnt/betacatenin is not involved in gastric carcinogenesis. However, the high frequency of survivin allows to suggest that other signaling pathways must be involved in the transformation of gastric tissue.
胃癌是全球第五大常见癌症，也是癌症相关死亡的第三大常见原因。据报道，在30%-50%的此类肿瘤中，Wnt/β-连环蛋白信号通路被激活。然而，该信号通路的失调尚未完全阐明。
确定E-钙黏蛋白、β-连环蛋白、腺瘤性息肉病基因（APC）、转录因子4（TCF-4）和生存素蛋白在胃腺癌组织中的表达，并与临床和病理参数相关联。
纳入71例行胃切除术的胃腺癌患者。采用免疫组织化学法检测E-钙黏蛋白、β-连环蛋白、APC、TCF-4和生存素蛋白的表达，并与临床和病理参数相关联。
E-钙黏蛋白在细胞膜上的表达率为3%；β-连环蛋白在细胞质和细胞核中的表达率分别为23.4%和3.1%；APC在细胞质中的表达率为94.6%；TCF-4在细胞核中的表达率为19.4%；生存素在细胞核中的表达率为93.9%。E-钙黏蛋白的表达率与老年患者相关（p=0.007），而β-连环蛋白与肿瘤<5 cm相关（p=0.041），APC与近端肿瘤相关（p=0.047）。此外，TCF-4在弥漫型（p=0.017）和T4期肿瘤（p=0.002）中的表达明显更高。
Wnt/β-连环蛋白信号通路不参与胃癌的发生。然而，生存素的高表达提示其他信号通路必定参与了胃组织的转化。
