Vicente-Carrillo Alejandro, Álvarez-Rodríguez Manuel, Rodríguez-Martínez Heriberto
Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Reprod Biol. 2017 Mar;17(1):69-78. doi: 10.1016/j.repbio.2017.01.001. Epub 2017 Jan 8.
The cation channel of sperm (CatSper) comprises four transmembrane subunits specifically expressed in human, equine, murine and ovine spermatozoa, apparently implicated in capacitation, hyperactivation and acrosome exocytosis. Western blotting and immunocytochemistry showed hereby that CatSper subunits are also present in boar spermatozoa, primarily over the sperm neck, tail and cytoplasmic droplets; albeit CatSper -1 presented in addition some distribution over the membrane of the acrosome and CatSper -2 and -4 over the membrane of the post-acrosome. The role of the Catsper channel in boar spermatozoa was investigated by extending the spermatozoa in media containing different calcium (Ca) availability and exposure to the capacitation-trigger bicarbonate, to progesterone or CatSper inhibitors (Mibefradil and NNC 55-0396), separately or sequentially, at physiological and toxicological doses. Extracellular Ca availability, combined with bicarbonate exposure (capacitation-inducing conditions) decreased sperm motility, similarly to when spermatozoa incubated in capacitation-inducing conditions was exposed to Mibefradil and NNC 55-0396. Exposure of these spermatozoa to progesterone did not cause significant changes in sperm motility and nor did it revert its decrease induced by CatSper antagonists. In conclusion, the CatSper channel regulates sperm motility during porcine capacitation-related events in vitro.
精子阳离子通道（CatSper）由四个跨膜亚基组成，在人类、马、小鼠和绵羊的精子中特异性表达，显然与获能、超活化和顶体胞吐作用有关。蛋白质免疫印迹法和免疫细胞化学法在此表明，CatSper亚基也存在于公猪精子中，主要分布在精子颈部、尾部和细胞质滴上；尽管CatSper -1在顶体膜上也有一些分布，CatSper -2和-4在后顶体膜上有分布。通过在含有不同钙（Ca）可用性的培养基中延长精子，并分别或依次以生理和毒理学剂量将其暴露于诱导获能的碳酸氢盐、孕酮或CatSper抑制剂（米贝拉地尔和NNC 55-0396），研究了CatSper通道在公猪精子中的作用。细胞外钙可用性与碳酸氢盐暴露（诱导获能条件）相结合会降低精子活力，这与在诱导获能条件下孵育的精子暴露于米贝拉地尔和NNC 55-0396时的情况类似。将这些精子暴露于孕酮不会导致精子活力发生显著变化，也不会逆转由CatSper拮抗剂诱导的活力下降。总之，CatSper通道在体外猪获能相关事件中调节精子活力。
