Yu Liang, Zhong Zhendong, Sun Hongbin, Yan Linxia, He Baomin, Li Supin, Ma Shuai, Yang Lili, Huang Yulan
Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
Institute of Laboratory Animals, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
Am J Transl Res. 2016 Dec 15;8(12):5211-5218. eCollection 2016.
Neuroglioma is the most common primary malignant tumor in neurosurgery. Due to unfavorable life quality of patients, the treatment of glioma is a major challenge in clinics. The search for effect treatment drugs thus benefits patient prognosis. As one derivative of resveratrol, pterostilbene has a wide spectrum of pharmaceutical functions, especially with the anti-tumor effects. This study thus investigated the effect of pterostilbene on neuroglioma and related mechanisms. U87 glioma cell line was divided into control, normal culture and different dosages of pterostilbene groups, which received 5 mM or 10 mM pterostilbene for 48 h. MTT assay was used to detect U87 cell proliferation, while invasion assay was employed to test the effect of pterostilbene on cell invasion, followed by flow cytometry assay for analyzing U87 cell apoptosis. Real-time PCR was used to test mRNA expression of Bcl-2 and Bax in glioma cells under the effect of pterostilbene, while Western blotting was used to detect alternation of Bcl-2 and Bax protein levels. Pterostilbene significantly inhibited proliferation and invasion abilities of glioma cells compared to those in control group (P<0.05). It can also enhance cell apoptosis, decrease mRNA and protein of Bcl-2 expression, and increase mRNA and protein expressions of Bax (P<0.05 compared to control group) in a dose-dependent manner. Pterostilbene can facilitate apoptosis of glioma cells, and inhibit their proliferation and invasion via mediating apoptotic/anti-apoptotic homeostasis.
神经胶质瘤是神经外科最常见的原发性恶性肿瘤。由于患者生活质量不佳，胶质瘤的治疗是临床上的一项重大挑战。因此，寻找有效的治疗药物有利于患者的预后。作为白藜芦醇的一种衍生物，紫檀芪具有广泛的药理作用，尤其是抗肿瘤作用。本研究因此探讨了紫檀芪对神经胶质瘤的作用及其相关机制。将U87胶质瘤细胞系分为对照组、正常培养组和不同剂量紫檀芪组，分别给予5 mM或10 mM紫檀芪处理48小时。采用MTT法检测U87细胞增殖，采用侵袭实验检测紫檀芪对细胞侵袭的影响，随后采用流式细胞术分析U87细胞凋亡。采用实时PCR检测紫檀芪作用下胶质瘤细胞中Bcl-2和Bax的mRNA表达，采用蛋白质印迹法检测Bcl-2和Bax蛋白水平的变化。与对照组相比，紫檀芪显著抑制了胶质瘤细胞的增殖和侵袭能力（P<0.05）。它还能增强细胞凋亡，降低Bcl-2表达的mRNA和蛋白水平，并以剂量依赖的方式增加Bax的mRNA和蛋白表达（与对照组相比，P<0.05）。紫檀芪可促进胶质瘤细胞凋亡，并通过介导凋亡/抗凋亡稳态抑制其增殖和侵袭。
