Li Chengbao, Huang Ying, Yao Xueya, Hu Baoji, Wu Suzhen, Chen Guannan, Lv Xin, Tian Fubo
Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of MedicineShanghai, China; Department of Medicine, Hebei North UniversityZhangjiakou, Hebei, China.
Jiangsu Province Key Laboratory of Anesthesiology and Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University Xuzhou, China.
Am J Transl Res. 2016 Dec 15;8(12):5557-5568. eCollection 2016.
This study aimed to investigate the protective effects and specific mechanisms of lugrandoside (LG) on lipopolysaccharides (LPS)-induced acute respiratory distress syndrome (ARDS). LG is a novel phenylpropanoid glycoside with many biological properties, isolated from the culinary leaves of Digitalis lutea L. and Digitalis grandiflora Miller. The primary indicators to assess the lung injury were infiltration of inflammatory cells; pulmonary edema; expression of proinflammatory cytokines, cyclo-oxygenase 2, and intracellular adhesion molecule 1; activation of nuclear factor-κB pathways; and cellular apoptosis. The results showed that LG evidently alleviated the inflammatory response, decreased the apoptosis of alveolar macrophages, and improved the lung injury in mice with LPS-induced ARDS. In conclusion, LG improved LPS-induced ARDS by anti-inflammation and anti-apoptosis and might be a promising pharmacological therapy for ARDS.
本研究旨在探讨鲁格兰糖苷（LG）对脂多糖（LPS）诱导的急性呼吸窘迫综合征（ARDS）的保护作用及具体机制。LG是一种具有多种生物学特性的新型苯丙素糖苷，从黄花毛地黄和大花毛地黄的烹饪叶中分离得到。评估肺损伤的主要指标包括炎性细胞浸润、肺水肿、促炎细胞因子、环氧化酶2和细胞间黏附分子1的表达、核因子-κB通路的激活以及细胞凋亡。结果表明，LG明显减轻了炎症反应，减少了肺泡巨噬细胞的凋亡，并改善了LPS诱导的ARDS小鼠的肺损伤。总之，LG通过抗炎和抗凋亡改善了LPS诱导的ARDS，可能是一种有前景的ARDS药物治疗方法。
