Athey Taryn B T, Vaillancourt Katy, Frenette Michel, Fittipaldi Nahuel, Gottschalk Marcelo, Grenier Daniel
Public Health Ontario Laboratory, Toronto, ON, Canada.
Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada.
Biomed Res Int. 2016;2016:6815894. doi: 10.1155/2016/6815894. Epub 2016 Dec 18.
Recently, we reported the purification and characterization of three distinct lantibiotics (named suicin 90-1330, suicin 3908, and suicin 65) produced by . In this study, we investigated the distribution of the three suicin lantibiotic gene clusters among serotype 2 strains belonging to sequence type (ST) 25 and ST28, the two dominant STs identified in North America. The genomes of 102 strains were interrogated for the presence of suicin gene clusters encoding suicins 90-1330, 3908, and 65. The gene cluster encoding suicin 65 was the most prevalent and mainly found among ST25 strains. In contrast, none of the genes related to suicin 90-1330 production were identified in 51 ST25 strains nor in 35/51 ST28 strains. However, the complete suicin 90-1330 gene cluster was found in ten ST28 strains, although some genes in the cluster were truncated in three of these isolates. The vast majority (101/102) of strains did not possess any of the genes encoding suicin 3908. In conclusion, this study indicates heterogeneous distribution of suicin genes in .
最近，我们报道了由……产生的三种不同羊毛硫抗生素（命名为suicin 90 - 1330、suicin 3908和suicin 65）的纯化及特性。在本研究中，我们调查了这三种suicin羊毛硫抗生素基因簇在属于序列类型（ST）25和ST28的2型菌株中的分布情况，这两种ST是在北美鉴定出的两个主要类型。对102株菌株的基因组进行检测，以确定是否存在编码suicin 90 - 1330、3908和65的suicin基因簇。编码suicin 65的基因簇最为普遍，主要存在于ST25菌株中。相比之下，在51株ST25菌株和35/51株ST28菌株中均未鉴定出与suicin 90 - 1330产生相关的任何基因。然而，在10株ST28菌株中发现了完整的suicin 90 - 1330基因簇，尽管其中3株分离株的该基因簇中的一些基因被截断。绝大多数（101/102）菌株不具备编码suicin 3908的任何基因。总之，本研究表明suicin基因在……中分布不均。
