National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-0046, Japan.
Hiroshima Research Center for Healthy Aging, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8530, Japan.
Appl Microbiol Biotechnol. 2017 Feb;101(4):1351-1357. doi: 10.1007/s00253-017-8098-7. Epub 2017 Jan 11.
S-Adenosylmethionine (SAM) is a key component of sulphur amino acid metabolism in living organisms and is synthesised from methionine and adenosine triphosphate by methionine adenosyltransferase. This molecule serves as the main biological methyl donor due to its active methylthio ether group. Notably, SAM has shown beneficial effects in clinical trials for the treatment of alcoholic liver disease, depression and joint pain. Due to the high potential value of SAM, current research efforts are attempting to develop a more rapid, cost-effective and higher yielding SAM production method than the conventional production system. In this mini-review, we describe the previously reported yeast gene that contributes to SAM accumulation by overexpression, mutation or deletion and summarise the genetic approach for the production of SAM in large industrial quantities.
S-腺苷甲硫氨酸（SAM）是生物体内硫氨基酸代谢的关键组成部分，由蛋氨酸和三磷酸腺苷通过蛋氨酸腺苷转移酶合成。由于其活性甲基硫醚基团，该分子是主要的生物甲基供体。值得注意的是，SAM 在治疗酒精性肝病、抑郁症和关节痛的临床试验中显示出了有益的效果。由于 SAM 的潜在价值很高，目前的研究工作正试图开发一种比传统生产系统更快、更具成本效益和更高产量的 SAM 生产方法。在这篇小型综述中，我们描述了以前报道的通过过表达、突变或缺失有助于 SAM 积累的酵母基因，并总结了用于大量工业生产 SAM 的遗传方法。
