Wang Zhengjia, Huang Ruiming, Sun Zhichao, Zhang Tong, Huang Jianqin
Nurturing Station for the State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an, Hangzhou, 311300, China.
Funct Integr Genomics. 2017 May;17(2-3):365-373. doi: 10.1007/s10142-016-0542-8. Epub 2017 Jan 11.
MicroRNAs (miRNAs) are important regulators of plant development and fruit formation. Mature embryos of hickory (Carya cathayensis Sarg.) nuts contain more than 70% oil (comprising 90% unsaturated fatty acids), along with a substantial amount of oleic acid. To understand the roles of miRNAs involved in oil and oleic acid production during hickory embryogenesis, three small RNA libraries from different stages of embryogenesis were constructed. Deep sequencing of these three libraries identified 95 conserved miRNAs with 19 miRNAs, 7 novel miRNAs (as well as their corresponding miRNAs), and 26 potentially novel miRNAs. The analysis identified 15 miRNAs involved in oil and oleic acid production that are differentially expressed during embryogenesis in hickory. Among them, nine miRNA sequences, including eight conserved and one novel, were confirmed by qRT-PCR. In addition, 145 target genes of the novel miRNAs were predicted using a bioinformatic approach. Our results provide a framework for better understanding the roles of miRNAs during embryogenesis in hickory.
微小RNA（miRNA）是植物发育和果实形成的重要调节因子。山核桃（Carya cathayensis Sarg.）坚果的成熟胚含有70%以上的油脂（其中90%为不饱和脂肪酸），以及大量的油酸。为了解miRNA在山核桃胚胎发生过程中参与油脂和油酸产生的作用，构建了来自胚胎发生不同阶段的三个小RNA文库。对这三个文库进行深度测序，鉴定出95个保守miRNA、19个miRNA*、7个新miRNA（及其相应的miRNA*）和26个潜在的新miRNA。分析确定了15个参与油脂和油酸产生的miRNA，它们在山核桃胚胎发生过程中差异表达。其中，9个miRNA序列（包括8个保守序列和1个新序列）通过qRT-PCR得到证实。此外，利用生物信息学方法预测了新miRNA的145个靶基因。我们的结果为更好地理解miRNA在山核桃胚胎发生过程中的作用提供了一个框架。
