Farias Simone, Mayer Diego A, de Oliveira Débora, de Souza Selene M A Guelli U, de Souza Antônio Augusto Ulson
Chemical and Food Engineering Department, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
Appl Biochem Biotechnol. 2017 Aug;182(4):1290-1306. doi: 10.1007/s12010-017-2399-2. Epub 2017 Jan 11.
The aim of this work was to remove the dyes Reactive Blue 221 (RB 221) and Reactive Blue 198 (RB 198) of synthetic effluent using the immobilized enzyme horseradish peroxidase (HRP) in Ca-alginate beads. Experimental parameters affecting the dye removal process such as the effect of pH, temperature, hydrogen peroxide concentration, mass capsules, and reuse were evaluated, and a numerical model of mass transfer was developed. A maximum removal of 93 and 75%, respectively, for the dyes RB 221 and RB 198, at pH 5.5 and temperature of 30 °C, concentration of hydrogen peroxide of 43.75 μM for dye RB 221 and 37.5 μM for the dye of RB 198 was obtained. A removal reaction of 180 min for RB 221 and 240 min for RB 198 was observed. Three reuse cycles of use of immobilized enzyme were achieved for both dyes. The numerical model proposed led to a good fit compared to experimental data. The HRP enzyme immobilized in Ca-alginate capsules showed a great potential for biotechnological applications, especially for the removal of reactive dyes.
这项工作的目的是使用固定在海藻酸钙珠粒中的辣根过氧化物酶（HRP）去除合成废水中的活性蓝221（RB 221）和活性蓝198（RB 198）染料。评估了影响染料去除过程的实验参数，如pH值、温度、过氧化氢浓度、胶囊质量和再利用情况，并建立了传质数值模型。在pH值为5.5、温度为30°C、RB 221染料的过氧化氢浓度为43.75μM、RB 198染料的过氧化氢浓度为37.5μM时，RB 221和RB 198染料的最大去除率分别为93%和75%。观察到RB 221的去除反应时间为180分钟，RB 198的去除反应时间为240分钟。两种染料均实现了固定化酶的三次重复使用循环。与实验数据相比，所提出的数值模型具有良好的拟合度。固定在海藻酸钙胶囊中的HRP酶在生物技术应用中显示出巨大潜力，特别是在去除活性染料方面。
