Weichselbaum R R, Dunphy E J, Beckett M A, Tybor A G, Moran W J, Goldman M E, Vokes E E, Panje W R
Department of Radiation and Cellular Oncology, Michael Reese/University of Chicago Center for Radiation Therapy, Illinois.
Head Neck. 1989 Sep-Oct;11(5):437-42. doi: 10.1002/hed.2880110510.
We studied epidermal growth factor receptor (EGFR) gene amplification and expression in 11 early passage human head and neck carcinoma cell lines. Three cell lines demonstrated EGFR gene amplification and 10 lines showed an increase in EGFR mRNA when compared with normal keratinocytes, placenta, and a human skin carcinoma cell line. The effects of EGF on growth in 6 head and neck carcinoma cell lines was also studied. Growth inhibition at a concentration of 20 ng/mL was observed in one cell line but had no effect on growth in 5 cell lines. An increase in EGFR may be important in the etiology of, or progression of, head and neck carcinoma although the mechanisms need to be elucidated by further study.
我们研究了11种早期传代的人头颈部癌细胞系中表皮生长因子受体（EGFR）基因的扩增及表达情况。与正常角质形成细胞、胎盘及一种人皮肤癌细胞系相比，3种细胞系显示出EGFR基因扩增，10种细胞系显示EGFR mRNA增加。我们还研究了表皮生长因子（EGF）对6种头颈部癌细胞系生长的影响。在一种细胞系中观察到20 ng/mL浓度的EGF对生长有抑制作用，但对5种细胞系的生长没有影响。EGFR增加在头颈部癌的病因学或进展过程中可能具有重要意义，尽管其机制有待进一步研究阐明。
