Issing W J, Heppt W J, Kastenbauer E R
Department of Otorhinolaryngology, University of Munich, Klinikum Grosshadern, Germany.
Eur Arch Otorhinolaryngol. 1993;250(7):392-5. doi: 10.1007/BF00180383.
Proto-oncogenes encoding growth factor receptors constitute several distinct families with close overall structural homology. The highest degree of homology can be observed in their catalytic domains, which are essential for intrinsic tyrosine kinase activity. Growth factor receptors in several of these families play critical roles in the regulation of normal cell growth and development. Some of these molecules have been implicated in the neoplastic process as well. A related DNA fragment distinct from epidermal growth factor receptor and erbB-2 genes was detected by reduced stringency hybridization of v-erbB to normal genomic human DNA. The expression of erbB-3 was studied by southern and northern blot technique in a subset of nine head and neck tumor cell lines, as well as in three immortalized cultures established from normal human salivary glands. No gene amplification of erb-B-3 was noted in any of the head and neck cell lines. The 6.2 kb transcript of erbB-3 was elevated significantly in an epidermoid carcinoma of the larynx (A388) and an esophageal carcinoma (HA 114).
编码生长因子受体的原癌基因构成了几个不同的家族，它们在整体结构上具有密切的同源性。在其催化结构域中可观察到最高程度的同源性，这些催化结构域对于内在酪氨酸激酶活性至关重要。其中几个家族中的生长因子受体在正常细胞生长和发育的调节中发挥着关键作用。其中一些分子也与肿瘤形成过程有关。通过v-erbB与正常人基因组DNA的低严谨度杂交，检测到一个与表皮生长因子受体和erbB-2基因不同的相关DNA片段。采用Southern和Northern印迹技术，在9种头颈肿瘤细胞系的一个亚组以及从正常人唾液腺建立的3种永生化培养物中研究了erbB-3的表达。在任何头颈细胞系中均未发现erb-B-3基因扩增。erbB-3的6.2 kb转录本在喉表皮样癌（A388）和食管癌（HA 114）中显著升高。
