cif毒力因子基因存在于铜绿假单胞菌角膜炎患者的分离菌株中。
The cif Virulence Factor Gene Is Present in Isolates From Patients With Pseudomonas aeruginosa Keratitis.
作者信息
Bahl Christopher D, St Laurent Jessica D, Karthikeyan R Siva Ganesa, Priya J Lakshmi, Prajna Lalitha, Zegans Michael E, Madden Dean R
机构信息
*Department of Biochemistry & Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH. Dr Bahl is now with the Department of Biochemistry, University of Washington, Seattle, WA, and Dr St. Laurent is now with the Department of Obstetrics and Gynecology, Brigham, and Women's Hospital, Boston, MA; †Department of Ocular Microbiology, Dr G. Venkatasamy Eye Research Institute, Aravind Medical Research Foundation, Madurai, India; ‡Department of Surgery (Ophthalmology), Geisel School of Medicine at Dartmouth, Hanover, NH; and §Department of Microbiology & Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH.
出版信息
Cornea. 2017 Mar;36(3):358-362. doi: 10.1097/ICO.0000000000001132.
PURPOSE
To determine whether the cif gene is present in pathogenic Pseudomonas aeruginosa isolates from patients with bacterial keratitis at Aravind Eye Hospital, a referral eye care center in southern India, and from corresponding environmental isolates.
METHODS
Polymerase chain reaction amplification was performed on strains of P. aeruginosa isolated from ocular infections and environmental soil samples were collected from the area surrounding Aravind Eye Hospital. DNA sequencing of 16S ribosomal DNA amplicons was performed to verify strain identity.
RESULTS
We determined that 45 of 48 patient isolates carry a genomic copy of cif. Analysis of a catalog of environmental strains previously isolated from the surrounding area revealed that only 4 of 10 P. aeruginosa strains and 1 of 14 strains of related species carry the cif gene.
CONCLUSIONS
This is the first study to show that P. aeruginosa strains with ocular pathogenicity carry the cif gene and that the presence of this gene may be enriched over its prevalence in the environment. Taken together, these results suggest a potential role for Cif in acute bacterial keratitis.
目的
确定在印度南部一家转诊眼科护理中心——阿拉文德眼科医院，从细菌性角膜炎患者中分离出的致病性铜绿假单胞菌菌株以及相应环境分离株中是否存在cif基因。
方法
对从眼部感染分离出的铜绿假单胞菌菌株进行聚合酶链反应扩增，并从阿拉文德眼科医院周边地区采集环境土壤样本。对16S核糖体DNA扩增子进行DNA测序以验证菌株身份。
结果
我们确定48株患者分离株中有45株携带cif的基因组拷贝。对先前从周边地区分离出的环境菌株目录进行分析发现，10株铜绿假单胞菌菌株中只有4株以及14株相关物种菌株中有1株携带cif基因。
结论
这是第一项表明具有眼部致病性的铜绿假单胞菌菌株携带cif基因且该基因的存在可能比其在环境中的流行率更高的研究。综上所述，这些结果表明Cif在急性细菌性角膜炎中可能发挥作用。
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