Woods Robbie S R, Keegan Helen, White Christine, Tewari Prerna, Toner Mary, Kennedy Susan, O'Regan Esther M, Martin Cara M, Timon Conrad V I, O'Leary John J
Department of Otolaryngology, St. James's Hospital, Dublin, Ireland.
Department of Otolaryngology, Trinity College Dublin, Dublin, Ireland.
Cancer Epidemiol Biomarkers Prev. 2017 May;26(5):702-710. doi: 10.1158/1055-9965.EPI-16-0619. Epub 2017 Jan 12.
Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (SCC) represents a distinct subgroup of head and neck tumors. We analyze the expression of cytokeratin 7, a junctional biomarker with a SEQIKA fragment, which stabilizes HPV-16 E7 transcripts, in oropharyngeal SCCs. Archived tumor specimens and epidemiologic data were collected from patients with oropharyngeal SCCs over 10 years. Briefly, DNA was extracted from tissue blocks, and HPV testing was carried out using SPF10 HPV PCR and INNO-LiPA HPV Genotyping. Immunohistochemical staining for CK7 and p16ink4a was performed on the Ventana BenchMark Ultra Immunostainer. Analysis was by light microscopy using the -score. CK7 expression was correlated with epidemiologic data, p16ink4a positivity, and HPV status using SPSS. CK7 expression was observed specifically and uniformly in the tonsillar crypt epithelium of normal tonsils and tumor specimens. There were 226 cases of oropharyngeal SCCs, with 70 demonstrating both HPV and p16 positivity. Of 216 cases evaluated for CK7, 106 demonstrated some positivity, whereas -score > 60 was seen in 55 of these. CK7 -score > 60 was significantly associated with tonsillar subsite and HPV and p16 positivity. An association between CK7 and HPV has been demonstrated. CK7-expressing tonsillar crypt cells potentially represent an oropharyngeal subsite susceptible to HPV-related SCC. Along with the cervix and anorectum, specific oropharyngeal expression of CK7 in a site predisposed to HPV-related tumors may suggest a role for CK7 in the pathogenesis of this subgroup of tumors. Further research is warranted to characterize the association between CK7 and HPV-related head and neck SCC. .
人乳头瘤病毒（HPV）相关的口咽鳞状细胞癌（SCC）是头颈部肿瘤中的一个独特亚组。我们分析了细胞角蛋白7的表达情况，细胞角蛋白7是一种具有SEQIKA片段的连接生物标志物，可稳定HPV - 16 E7转录本，在口咽SCC中进行分析。在10年期间收集了口咽SCC患者的存档肿瘤标本和流行病学数据。简要来说，从组织块中提取DNA，并使用SPF10 HPV PCR和INNO - LiPA HPV基因分型进行HPV检测。在Ventana BenchMark Ultra免疫染色仪上对CK7和p16ink4a进行免疫组织化学染色。通过光学显微镜使用-score进行分析。使用SPSS将CK7表达与流行病学数据、p16ink4a阳性以及HPV状态相关联。在正常扁桃体和肿瘤标本的扁桃体隐窝上皮中特异性且均匀地观察到CK7表达。有226例口咽SCC病例，其中70例同时显示HPV和p16阳性。在评估CK7的216例病例中，106例显示出一定程度的阳性，其中55例-score>60。CK7 - score>60与扁桃体亚部位以及HPV和p16阳性显著相关。已证实CK7与HPV之间存在关联。表达CK7的扁桃体隐窝细胞可能代表易患HPV相关SCC的口咽亚部位。除了子宫颈和直肠，CK7在易患HPV相关肿瘤的部位的特异性口咽表达可能表明CK7在该肿瘤亚组的发病机制中起作用。有必要进行进一步研究以表征CK7与HPV相关的头颈部SCC之间的关联。
