Li Jing-Jian, Xiong Chao, Liu Yue, Liang Jun-Song, Zhou Xing-Wen
College of Forestry and Landscape Architecture, South China Agricultural University Guangzhou, China.
College of Pharmacy, Hubei University of Chinese Medicine Wuhan, China.
Front Plant Sci. 2016 Dec 26;7:1956. doi: 10.3389/fpls.2016.01956. eCollection 2016.
Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application.
正确识别药用植物成分对于其安全使用以及草药供应链的监管至关重要。环介导等温扩增技术（LAMP）是一种最近开发的用于鉴定草药种类的方法。这种新颖的分子生物学技术能够实现及时且准确的检测，特别是在缺乏支持聚合酶链式反应设施的基础设施的环境中。使用该方法的研究改变了我们对草药鉴定的范围和复杂性的看法。在本综述中，我们介绍了LAMP分析，涵盖了LAMP分析方法开发的基本原理和重要方面。然后我们对基于LAMP的方法在检测和鉴定生药用植物材料及其加工产品中的应用进行了批判性综述。我们还提供了在草药鉴定中使用LAMP方案的实用标准操作规程（SOP），并考虑了LAMP技术在草药鉴定未来发展中的前景及其应用所面临的挑战。
