Song Weidu, Zhang Taiyi, Lin Huichao, Yang Yujing, Zhao Gaozhen, Huang Xiaowen
State Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, China.
Micromachines (Basel). 2022 Apr 17;13(4):636. doi: 10.3390/mi13040636.
Nucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the limitations of this technology, it is not capable of meeting the needs of large-scale rapid detection. To solve this problem, many new techniques for detecting nucleic acids of SARS-CoV-2 have been reported. Therefore, a review that systematically and comprehensively introduces and compares various detection technologies is needed. In this paper, we not only review the traditional NAT but also provide an overview of microfluidic-based NAT technologies and summarize and discuss the characteristics and development prospects of these techniques.
核酸检测（NAT）在疫情期间对遏制SARS-CoV-2的传播起到了关键作用。目前，政府和医学委员会采用金标准技术——定量实时聚合酶链反应（qRT-PCR）技术来检测SARS-CoV-2。由于该技术存在局限性，无法满足大规模快速检测的需求。为解决这一问题，已报道了许多检测SARS-CoV-2核酸的新技术。因此，需要一篇系统全面地介绍和比较各种检测技术的综述。在本文中，我们不仅回顾了传统的核酸检测技术，还概述了基于微流控的核酸检测技术，并总结和讨论了这些技术的特点和发展前景。
