Su Nana, Wu Qi, Cui Jin
College of Life Sciences, Nanjing Agricultural UniversityNanjing, China; School of Land and Food, University of Tasmania, HobartTAS, Australia.
College of Life Sciences, Nanjing Agricultural University Nanjing, China.
Front Plant Sci. 2016 Dec 26;7:1976. doi: 10.3389/fpls.2016.01976. eCollection 2016.
Effects of nitrogen (N) deficiency and sucrose (Suc) addition on regulation of anthocyanin biosynthesis and their relationship were investigated in this study. Radish sprouts subjected to N deficiency had 50% higher anthocyanin accumulation than when grown in Hoagland solution (a nutrient medium with all macronutrients). The contents of endogenous soluble sugars (Suc, fructose, and glucose) in the hypocotyls were also markedly increased by N limitation, with Suc showing the highest increase. Inhibition of carbohydrate biosynthesis by addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) also eliminated N deficiency-induced anthocyanin accumulation. The latter was further supported by the expression of anthocyanin biosynthesis related genes and decreased activities of nitrate reductase in the presence of Suc. Together our results indicate that N deficiency-induced anthocyanin accumulation was, at least partly, dependent on the increase of the soluble sugar, especially Suc. This work is the first comprehensive study on relationship between N deficiency and sugar content on anthocyanin accumulation in the hypocotyls of radish sprouts.
本研究调查了缺氮和添加蔗糖对花青素生物合成调控的影响及其关系。与在霍格兰溶液（一种含有所有大量营养素的营养培养基）中生长相比，缺氮条件下的萝卜芽花青素积累量高出50%。缺氮也显著增加了下胚轴中内源可溶性糖（蔗糖、果糖和葡萄糖）的含量，其中蔗糖增加幅度最大。添加3-(3,4-二氯苯基)-1,1-二甲基脲（DCMU）抑制碳水化合物生物合成也消除了缺氮诱导的花青素积累。花青素生物合成相关基因的表达以及在蔗糖存在下硝酸还原酶活性的降低进一步支持了后者。我们的研究结果共同表明，缺氮诱导的花青素积累至少部分依赖于可溶性糖尤其是蔗糖的增加。这项工作是关于缺氮和糖含量对萝卜芽下胚轴花青素积累影响之间关系的首次全面研究。
