Hashimoto Y, Sakaizumi M, Nakamura Y, Moriwaki K, Yamakawa T, Suzuki A
Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science.
J Biochem. 1989 Aug;106(2):319-22. doi: 10.1093/oxfordjournals.jbchem.a122851.
C57BL/10(H-2b) and A/J(H-2a) mice have different phenotypes as to the age dependent changes in the expression of GM1 and GD1a in the liver, i.e., the contents of these two components in total gangliosides in C57BL/10 and A/J mice were 1 and 20% at 4 wk and had decreased to almost 0 and 10%, respectively, at 8 wk [Y. Nakamura et al. (1988) J. Biochem. 103, 393-396]. To analyze the mode of inheritance of these phenotypes, C57BL/10 mice were mated with A/J or A/Wy(H-2a) mice. The phenotype of A/J or A/Wy mice was found to be inherited by the F1 hybrids, indicating that the phenotype of A mice is dominant over that of C57BL/10 mice. The (C57BL/10 x A/Wy)F1 hybrids were then backcrossed with one of the parent strains, C57BL/10 mice. The phenotype of A/Wy mice was demonstrated to be inherited by 23 out of 35 mice and all of the 23 had the H-2a haplotype. These results suggest that the two phenotypes of C57BL/10 and A mice are controlled by a pair of allelic genes on a locus which is closely linked to the H-2 complex on chromosome 17. Unexpectedly, B10. A(H-2a) mice were found to have significantly higher amounts of GM1 and GD1a than both C57BL/10 and A mice, although they were a H-2 congenic strain carrying the H-2 genes transferred from A/Wy mice and should have the same genes on loci next to H-2 as one of the two strains. It remains to be determined how B10.A mice have higher amounts of GM1 and GD1a than C57BL/10 and A/Wy mice.
C57BL/10（H-2b）和A/J（H-2a）小鼠在肝脏中GM1和GD1a表达的年龄依赖性变化方面具有不同的表型，即C57BL/10和A/J小鼠总神经节苷脂中这两种成分的含量在4周时分别为1%和20%，而在8周时分别降至几乎0%和10%[Y. Nakamura等人（1988年）《生物化学杂志》103卷，393 - 396页]。为了分析这些表型的遗传模式，将C57BL/10小鼠与A/J或A/Wy（H-2a）小鼠交配。发现A/J或A/Wy小鼠的表型由F1杂种继承，这表明A小鼠的表型对C57BL/10小鼠的表型呈显性。然后将（C57BL/10×A/Wy）F1杂种与亲本菌株之一C57BL/10小鼠回交。结果表明，35只小鼠中有23只继承了A/Wy小鼠的表型，并且这23只小鼠都具有H-2a单倍型。这些结果表明，C57BL/10和A小鼠的两种表型由位于与17号染色体上H-2复合体紧密连锁的位点上的一对等位基因控制。出乎意料的是，尽管B10.A（H-2a）小鼠是携带从A/Wy小鼠转移而来的H-2基因的H-2同基因品系，并且在H-2旁边的位点上应该与这两个菌株之一具有相同的基因，但发现其GM1和GD1a的含量显著高于C57BL/10和A小鼠。B10.A小鼠的GM1和GD1a含量高于C57BL/10和A/Wy小鼠的原因仍有待确定。
