Varmazyar Sajad, Marashi Sayed Mahdi, Shoja Zabihollah, Tornesello Maria Lina, Buonaguro Franco M, Shahmahmoodi Shohreh, Safaie-Naraghi Zahra, Jalilvand Somayeh
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 14155, Iran.
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
Med Microbiol Immunol. 2017 Apr;206(2):157-163. doi: 10.1007/s00430-016-0491-9. Epub 2017 Jan 12.
A single-nucleotide polymorphism (SNP) in the promoter region of MDM2 (SNP309T>G, rs2279744) has been shown to increase the expression of the MDM2 protein in various cancer types. However, only one study has analyzed the role of the MDM2 polymorphism in the development of Kaposi's sarcoma (KS). The association of MDM2 SNP309 with classic KS risk was evaluated in 79 Iranian patients with classic KS and 123 healthy controls. The MDM2 SNP309 was genotyped using PCR and restriction fragment length polymorphism methods. No significant correlation was found between the SNP309 polymorphism in MDM2 promoter and classic KS risk. There was no significant correlation between gender and disease stage. However, a significant association was found between SNP309 GG genotype and younger age (≤50 years) (odds ratio 9.5, 95% confidence intervals 1.5-60, p = 0.03). Our findings support no major role for the MDM2 SNP309 in KS development although it might influence the clinical outcome of KS in younger patients.
MDM2启动子区域的单核苷酸多态性（SNP）（SNP309T>G，rs2279744）已被证明可增加多种癌症类型中MDM2蛋白的表达。然而，仅有一项研究分析了MDM2多态性在卡波西肉瘤（KS）发生发展中的作用。对79例伊朗经典型KS患者和123例健康对照者评估了MDM2 SNP309与经典型KS风险的相关性。采用聚合酶链反应（PCR）和限制性片段长度多态性方法对MDM2 SNP309进行基因分型。未发现MDM2启动子中的SNP309多态性与经典型KS风险之间存在显著相关性。性别与疾病分期之间也无显著相关性。然而，发现SNP309 GG基因型与较年轻年龄（≤50岁）之间存在显著关联（比值比9.5，95%置信区间1.5 - 60，p = 0.03）。我们的研究结果支持MDM2 SNP309在KS发生发展中无主要作用，尽管它可能影响年轻患者KS的临床结局。
