School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Virus Res. 2012 Feb;163(2):644-9. doi: 10.1016/j.virusres.2011.09.027. Epub 2011 Sep 22.
Kaposi's sarcoma (KS) is a rare cancer in Iran and there is no epidemiological and molecular information about HHV-8 variants circulating among the Iranian population. In this study HHV-8 sequences have been analyzed in 43 cutaneous KS biopsies from Iranian patients mainly affected by classic KS. DNA samples were subjected to PCR amplification of HHV-8 ORF26, T0.7 and K1 followed by direct nucleotide sequencing and phylogenetic analysis. The analysis of ORF26 showed that 30 (69.8%) and 13 (30.2%) samples belonged to subtypes A/C and K, respectively. In general, the clustering of HHV-8 T0.7 variants paralleled that of ORF26. Genotyping of K1 sequences showed that the majority of samples (39 out of 41) fall into the large C clade with only 2 belonging to the A clade. In conclusion, HHV-8 variants identified among classic Iranian KS are largely related to Eurasian genotypes previously identified in KS from Mediterranean, Middle East, and East Asian regions.
卡波西肉瘤（KS）在伊朗较为罕见，有关在伊朗人群中流行的 HHV-8 变异体的流行病学和分子信息尚不清楚。本研究分析了来自主要受经典 KS 影响的 43 例伊朗皮肤 KS 活检患者的 HHV-8 序列。将 DNA 样本进行 HHV-8 ORF26、T0.7 和 K1 的 PCR 扩增，然后进行直接核苷酸测序和系统发育分析。ORF26 的分析表明，30 份（69.8%）和 13 份（30.2%）样本分别属于亚型 A/C 和 K。总体而言，HHV-8 T0.7 变异体的聚类与 ORF26 相似。K1 序列的基因分型表明，大多数样本（41 份中的 39 份）属于大型 C 分支，只有 2 份属于 A 分支。总之，在经典伊朗 KS 中鉴定的 HHV-8 变异体与先前在来自地中海、中东和东亚地区的 KS 中鉴定的欧亚基因型密切相关。
