Lago Larissa, Nunes Emilene A, Vigato Aryane A, Souza Vanessa C O, Barbosa Fernando, Sato João R, Batista Bruno L, Cerchiaro Giselle
Center for Natural Sciences and Humanities, Universidade Federal do ABC - UFABC, Santo André, SP, Brazil.
Department of Clinical Analysis, Toxicological and Bromatological, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Ribeirão Prêto, Brazil.
Biometals. 2017 Feb;30(1):83-96. doi: 10.1007/s10534-016-9988-3. Epub 2017 Jan 12.
Essential trace elements are commonly found in altered concentrations in the brains of patients with neurodegenerative diseases. Many studies in trace metal determination and quantification are conducted in tissue, cell culture or whole brain. In the present investigation, we determined by ICP-MS Fe, Cu, Zn, Ca, Se, Co, Cr, Mg, and Mn in organelles (mitochondria, nuclei) and whole motor neuron cell cultured in vitro. We performed experiments using two ways to access oxidative stress: cell treatments with HO or Aβ-42 peptide in its oligomeric form. Both treatments caused accumulation of markers of oxidative stress, such as oxidized proteins and lipids, and alteration in DNA. Regarding trace elements, cells treated with HO showed higher levels of Zn and lower levels of Ca in nuclei when compared to control cells with no oxidative treatments. On the other hand, cells treated with Aβ-42 peptide in its oligomeric form showed higher levels of Mg, Ca, Fe and Zn in nuclei when compared to control cells. These differences showed that metal flux in cell organelles during an intrinsic external oxidative condition (HO treatment) are different from an intrinsic external neurodegenerative treatment.
必需微量元素在神经退行性疾病患者大脑中的浓度通常会发生改变。许多关于痕量金属测定和定量的研究是在组织、细胞培养物或整个大脑中进行的。在本研究中，我们通过电感耦合等离子体质谱法（ICP-MS）测定了体外培养的运动神经元细胞器（线粒体、细胞核）和整个运动神经元细胞中的铁（Fe）、铜（Cu）、锌（Zn）、钙（Ca）、硒（Se）、钴（Co）、铬（Cr）、镁（Mg）和锰（Mn）。我们采用两种方法引发氧化应激进行实验：用羟基自由基（HO）或寡聚形式的Aβ-42肽处理细胞。两种处理均导致氧化应激标志物（如氧化的蛋白质和脂质）的积累以及DNA的改变。关于微量元素，与未进行氧化处理的对照细胞相比，用HO处理的细胞细胞核中锌含量更高，钙含量更低。另一方面，与对照细胞相比，用寡聚形式的Aβ-42肽处理的细胞细胞核中镁、钙、铁和锌的含量更高。这些差异表明，在内在外部氧化条件（HO处理）下细胞器中的金属通量与内在外部神经退行性处理下的不同。
