Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing 100193, China.
Sci Rep. 2017 Jan 13;7:40819. doi: 10.1038/srep40819.
Sweet potato is an important food and bio-energy crop, and investigating the mechanisms underlying salt tolerance will provide information for salt-tolerant breeding of this crop. Here, the root transcriptomes of the salt-sensitive variety Lizixiang and the salt-tolerant line ND98 were compared to identify the genes and pathways involved in salt stress responses. In total, 8,744 and 10,413 differentially expressed genes (DEGs) in Lizixiang and ND98, respectively, were involved in salt responses. A lower DNA methylation level was detected in ND98 than in Lizixiang. In both genotypes, the DEGs, which function in phytohormone synthesis and signalling and ion homeostasis, may underlie the different degrees of salt tolerance. Significant up-regulations of the genes involved in the jasmonic acid (JA) biosynthesis and signalling pathways and ion transport, more accumulation of JA, a higher degree of stomatal closure and a lower level of Na were found in ND98 compared to Lizixiang. This is the first report on transcriptome responses to salt tolerance in sweet potato. These results reveal that the JA signalling pathway plays important roles in the response of sweet potato to salt stress. This study provides insights into the mechanisms and genes involved in the salt tolerance of sweet potato.
甘薯是一种重要的粮食和生物能源作物，研究其耐盐机制可为该作物的耐盐育种提供信息。本研究比较了盐敏感品种丽子香和耐盐系 ND98 的根转录组，以鉴定参与盐胁迫响应的基因和途径。在丽子香和 ND98 中，分别有 8744 个和 10413 个差异表达基因（DEGs）参与盐响应。ND98 的 DNA 甲基化水平低于丽子香。在两种基因型中，参与植物激素合成和信号转导以及离子稳态的 DEGs 可能是导致不同耐盐程度的基础。与丽子香相比，ND98 中参与茉莉酸（JA）生物合成和信号转导以及离子转运的基因显著上调，JA 积累更多，气孔关闭程度更高，Na 含量更低。这是首次报道甘薯对耐盐性的转录组响应。这些结果表明，JA 信号通路在甘薯对盐胁迫的响应中发挥重要作用。本研究为甘薯耐盐性的机制和基因提供了新的见解。
