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二氢乳清酸脱氢酶荧光分析法，有望成为一种癌症生物标志物。

Fluorescence assay of dihydroorotate dehydrogenase that may become a cancer biomarker.

机构信息

Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan.

Department of Pharmacy, School of Medicine, Shenzhen University, 3688 Nanhai Boulevard, Shenzhen, Guangdong 518060, China.

出版信息

Sci Rep. 2017 Jan 13;7:40670. doi: 10.1038/srep40670.

DOI:10.1038/srep40670
PMID:28084471
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5233952/
Abstract

We developed an assay method for measuring dihydroorotate dehydrogenase (DHODH) activity in cultured HeLa cells and fibroblasts, and in stage III stomach cancer and adjacent normal tissues from the same patient. The assay comprised enzymatic reaction of DHODH with a large amount of dihydroorotic acid substrate, followed by fluorescence (FL) detection specific for orotic acid using the 4-trifluoromethyl-benzamidoxime fluorogenic reagent. The DHODH activities in the biologically complex samples were readily measured by the assay method. Our data indicate significantly higher DHODH activity in HeLa cells (340 ± 25.9 pmol/10 cells/h) than in normal fibroblasts (54.1 ± 7.40 pmol/10 cells/h), and in malignant tumour tissue (1.10 ± 0.19 nmol/mg total proteins/h) than in adjacent normal tissue (0.24 ± 0.11 nmol/mg total proteins/h). This is the first report that DHODH activity may be a diagnostic biomarker for cancer.

摘要

我们开发了一种用于测量培养的 HeLa 细胞和成纤维细胞以及来自同一患者的 III 期胃癌和相邻正常组织中二氢乳清酸脱氢酶 (DHODH) 活性的测定方法。该测定法包括 DHODH 与大量二氢乳清酸底物的酶促反应，然后使用 4-三氟甲基苯甲酰胺肟荧光试剂对乳清酸进行特异性荧光 (FL) 检测。通过该测定方法可以轻松测量生物复杂样品中的 DHODH 活性。我们的数据表明，HeLa 细胞中的 DHODH 活性（340±25.9 pmol/10 细胞/h）明显高于正常成纤维细胞（54.1±7.40 pmol/10 细胞/h），恶性肿瘤组织中的 DHODH 活性（1.10±0.19 nmol/mg 总蛋白/h）明显高于相邻正常组织（0.24±0.11 nmol/mg 总蛋白/h）。这是 DHODH 活性可能成为癌症诊断生物标志物的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/ed21e1be8fb9/srep40670-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/128e176afe32/srep40670-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/bb3e92a0a40f/srep40670-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/175d76400c12/srep40670-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/a82fc286bcb0/srep40670-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/ed21e1be8fb9/srep40670-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/128e176afe32/srep40670-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/ea5b3280250e/srep40670-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/b6eae77c5aaf/srep40670-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/bb3e92a0a40f/srep40670-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/175d76400c12/srep40670-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/a82fc286bcb0/srep40670-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe04/5233952/ed21e1be8fb9/srep40670-f7.jpg

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