Shie Jiun-Jie, Liu Ying-Chih, Hsiao Jye-Chian, Fang Jim-Min, Wong Chi-Huey
Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan.
Genomics Research Center, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan.
Chem Commun (Camb). 2017 Jan 26;53(9):1490-1493. doi: 10.1039/c6cc08805h.
A new fluorescence-forming probe, coumOCT, designed by fusing cyclooctyne with a coumarin fluorophore was successfully used for the imaging of azido-glycoconjugates in living HeLa cells. This probe is cell-permeable and generates fluorescence after triazole formation, thus minimizing the background signal and enabling the real-time intracellular imaging of glycoconjugate trafficking.
一种新的荧光形成探针coumOCT，通过将环辛炔与香豆素荧光团融合设计而成，已成功用于活的HeLa细胞中叠氮糖缀合物的成像。该探针具有细胞渗透性，在形成三唑后产生荧光，从而将背景信号降至最低，并能够对糖缀合物运输进行实时细胞内成像。
